Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
Chemistry. 2012 Apr 10;18(15):4568-74. doi: 10.1002/chem.201102947. Epub 2012 Feb 28.
A new atomic force microscopy (AFM)-based chemo-mechanical tweezer has been developed that can measure mechanical properties of individual macromolecules in supramolecular assembly and reveal positions of azide-containing polymers. A key feature of the new technology is the use of an AFM tip densely modified with 4-dibenzocyclooctynols (chemo-mechanical tweezer) that can react with multiple azide containing macromolecules of micelles to give triazole "clicked" compounds, which during retracting phases of AFM imaging are removed from the macromolecular assembly thereby providing a surface topographical image and positions of azide-containing polymers. The force-distance curves gave mechanical properties of removal of individual molecules from a supramolecular assembly. The new chemo-mechanical tweezer will make it possible to characterize molecular details of macromolecular assemblies thereby offering new avenues to tailor properties of such assemblies.
一种新的基于原子力显微镜（AFM）的化学机械镊子已经被开发出来，它可以测量超分子组装中单分子的机械性能，并揭示含叠氮聚合物的位置。这项新技术的一个关键特点是使用了一个密集修饰有 4-二苯并环辛炔醇（化学机械镊子）的 AFM 尖端，它可以与胶束中的多个含叠氮的大分子反应，生成三唑“点击”化合物，在 AFM 成像的缩回阶段，这些化合物从大分子组装体中被去除，从而提供了一个表面形貌图像和含叠氮聚合物的位置。力-距离曲线给出了从超分子组装体中去除单个分子的机械性能。新的化学机械镊子将使我们能够对大分子组装体的分子细节进行表征，从而为这些组装体的性质定制提供新的途径。
