Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Angew Chem Int Ed Engl. 2012 Apr 2;51(14):3382-5. doi: 10.1002/anie.201108710. Epub 2012 Feb 28.
All bound together: self-assembled symmetric DNA polyhedra were used to organize proteins in 3D space. Biotin moieties were incorporated into the self-assembled symmetric DNA polyhedra. Upon incubation with streptavidin (STV) protein, an STV protein became bound to each polyhedral face, thus resulting in well-structured DNA polyhedra/STV complexes. This strategy was also applied to different 3D DNA nanostructures and different proteins.
自组装对称 DNA 多面体被用来在 3D 空间中组织蛋白质。生物素部分被掺入自组装对称 DNA 多面体中。与链霉亲和素（STV）蛋白孵育后，每个多面体表面都结合了一个 STV 蛋白，从而形成结构良好的 DNA 多面体/STV 复合物。该策略还应用于不同的 3D DNA 纳米结构和不同的蛋白质。
