Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, Colorado, USA.
Clin Transl Sci. 2012 Feb;5(1):21-6. doi: 10.1111/j.1752-8062.2011.00378.x. Epub 2012 Feb 23.
The experimental aims of this study were to determine: (1) whether nitric oxide-mediated endothelium-dependent vasodilation is blunted in adult humans with borderline high plasma low-density lipoprotein (LDL)-cholesterol compared with adults with optimal/near optimal LDL-cholesterol levels; and, if so: (2) whether the magnitude of impairment in adults with borderline high LDL-cholesterol is similar to adults with high LDL-cholesterol. Forearm blood flow responses to intraarterial infusions of acetylcholine and sodium nitroprusside were measured in 50 middle-aged (43-64 year) adults: 20 in the optimal/near optimal LDL-cholesterol range (<130 mg/dL); 20 with borderline high LDL-cholesterol (130-159 mg/dL); and 10 with high LDL-cholesterol ($160 mg/dL). In addition, blood flow responses to acetylcholine were determined in the absence and presence of the endothelial nitric oxide synthase inhibitor N(G) -monomethyl-L-arginine (L-NMMA). Vasodilation to acetylcholine was 20% lower (p < 0.05) in the borderline high (from 4.3 ± 0.2 to 12.3 ± 0.8 mL/100 mL tissue/min) and high (from 4.3 ± 0.3 to 12.0 ± 0.5 mL/100 mL tissue/min) LDL-cholesterol groups compared with the optimal/near optimal (from 4.4 ± 0.2 to 14.5 ± 0.5 mL/100 mL tissue/min) LDL-cholesterol group. L-NMMA significantly reduced (30%) the vasodilator response to acetylcholine in the optimal/near optimal LDL-cholesterol group but not the borderline high or high LDL-cholesterol groups. Borderline high LDL-cholesterol is associated with impaired nitric oxide-mediated endothelium-dependent vasodilation.
(1)与 LDL 胆固醇水平最佳/接近最佳的成年人相比，边缘性高血浆 LDL 胆固醇（LDL-C）的成年人体内一氧化氮介导的内皮依赖性血管舒张是否减弱；如果是这样：(2) 边缘性高 LDL-C 成年人的损伤程度是否与高 LDL-C 成年人相似。在 50 名中年（43-64 岁）成年人中测量了前臂血流对乙酰胆碱和硝普钠的动脉内输注的反应：20 名处于最佳/接近最佳 LDL-C 范围内（<130mg/dL）；20 名有边缘性高 LDL-C（130-159mg/dL）；10 名有高 LDL-C（$160mg/dL）。此外，在不存在和存在内皮型一氧化氮合酶抑制剂 N(G)-单甲基-L-精氨酸（L-NMMA）的情况下，还确定了对乙酰胆碱的血流反应。在边缘性高（从 4.3±0.2 到 12.3±0.8mL/100mL 组织/分钟）和高（从 4.3±0.3 到 12.0±0.5mL/100mL 组织/分钟）LDL-C 组中，乙酰胆碱诱导的血管舒张作用比最佳/接近最佳（从 4.4±0.2 到 14.5±0.5mL/100mL 组织/分钟）LDL-C 组低约 20%（p<0.05）。L-NMMA 显著降低（~30%）了最佳/接近最佳 LDL-C 组中乙酰胆碱的血管舒张反应，但对边缘性高或高 LDL-C 组没有影响。边缘性高 LDL-C 与一氧化氮介导的内皮依赖性血管舒张受损有关。
