College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China.
Vet Parasitol. 2012 Jul 6;187(3-4):534-7. doi: 10.1016/j.vetpar.2012.02.002. Epub 2012 Feb 9.
The prevalence of Cryptosporidium spp. was investigated in scale quail (Coturnix coturnix japonica) farms in Henan Province, China between September 2006 and August 2007. One thousand eight hundred and eighteen fecal samples from 47 quail farms in five areas were collected for the examination of Cryptosporidium oocysts. The overall prevalence of Cryptosporidium was 13.1% (95% CI 13.1±1.6%) (29 of 47 farms), with 72-100-day-old quails having the highest prevalence (23.6%, 95% CI 23.6±2.6%) (χ(2)=64.91; ρ<0.01). The highest prevalence was observed in autumn (21.8%, 95% CI 21.8±3.1%) and the lowest in winter (χ(2)=74.83; ρ<0.01). Two hundred and thirty-nine Cryptosporidium-positive samples were analyzed by PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene, and 42 were further analyzed by DNA sequencing of the PCR products. Two Cryptosporidium species were identified, Cryptosporidium baileyi in 237 birds on 29 farms, and potentially zoonotic Cryptosporidium meleagridis in only two birds on two farms. These findings may suggest that quails are not a major source of zoonotic Cryptosporidium in the study area.
2006 年 9 月至 2007 年 8 月期间，在中国河南省的养殖鹌鹑场调查了隐孢子虫属的流行情况。从五个地区的 47 个鹌鹑场采集了 1818 份粪便样本，用于检查隐孢子虫卵囊。隐孢子虫的总流行率为 13.1%（95%置信区间 13.1±1.6%）（29/47 个农场），72-100 日龄鹌鹑的流行率最高（23.6%，95%置信区间 23.6±2.6%）（χ(2)=64.91；ρ<0.01）。秋季的流行率最高（21.8%，95%置信区间 21.8±3.1%），冬季的流行率最低（χ(2)=74.83；ρ<0.01）。通过 PCR-限制性片段长度多态性（RFLP）分析小亚基（SSU）rRNA 基因对 239 份隐孢子虫阳性样本进行分析，对 42 份 PCR 产物进行 DNA 测序进一步分析。鉴定出两种隐孢子虫，29 个农场的 237 只鸟中存在隐孢子虫贝利种，仅在两个农场的两只鸟中存在潜在的人兽共患隐孢子虫美莱格利迪斯种。这些发现表明鹌鹑可能不是该研究地区人兽共患隐孢子虫的主要来源。
