L'Oréal Research and Innovation, Clichy, France.
Exp Dermatol. 2012 Mar;21(3):205-10. doi: 10.1111/j.1600-0625.2011.01434.x.
A proteomic analysis of stratum corneum (SC) samples of normal healthy skin revealed the presence of more than 70 proteins by 2D electrophoresis. The majority of these proteins to our knowledge have not yet been described in normal SC. We analysed by Western blot the levels of 25 proteins in the SC taken from postmenopausal and dry skin compared with young and normal skin, respectively. In postmenopausal skin, there was a significantly increased amount of heat shock protein 27, plakoglobin and desmoglein 1, whereas transglutaminase 3, apolipoprotein D and acid ceramidase levels were significantly reduced compared with the SC of young skin. We confirmed corneodesmosin as a marker of dry skin. In addition, we showed for the first time that the levels of both phosphatidylethanolamine-binding protein 1 and annexin A2 were significantly increased in the SC of dry skin compared with the SC of normal skin. These results suggest that a proteomic analysis of the SC obtained using a non-invasive varnish stripping method is an attractive alternative to invasive methods to better characterize changes in the physiology of ageing and dry skin.
对正常健康皮肤的角质层（SC）样本进行蛋白质组学分析，通过二维电泳发现了 70 多种蛋白质。据我们所知，这些蛋白质中的大多数在正常 SC 中尚未被描述。我们通过 Western blot 分析了绝经后和干燥皮肤的 SC 中的 25 种蛋白质的水平，分别与年轻和正常皮肤进行了比较。在绝经后皮肤中，热休克蛋白 27、桥粒斑蛋白和桥粒芯糖蛋白 1 的含量显著增加，而转谷氨酰胺酶 3、载脂蛋白 D 和酸性神经酰胺酶的水平与年轻皮肤的 SC 相比则显著降低。我们确认了角蛋白丝结合蛋白 1 是干燥皮肤的标志物。此外，我们首次表明，与正常皮肤的 SC 相比，干燥皮肤的 SC 中磷酸乙醇胺结合蛋白 1 和膜联蛋白 A2 的水平显著增加。这些结果表明，使用非侵入性指甲油剥落方法获得的 SC 的蛋白质组学分析是一种有吸引力的替代侵入性方法，可更好地描述衰老和干燥皮肤生理变化。
