Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA.
J Fish Biol. 2012 Mar;80(3):538-54. doi: 10.1111/j.1095-8649.2011.03185.x. Epub 2012 Feb 3.
The role of the C24 sulphate in the mating pheromone component, 7α,12α,24-trihydroxy-5α-cholan-3-one 24-sulphate (3kPZS), to specifically induce upstream movement in ovulated female sea lampreys Petromyzon marinus was investigated. 7α,12α-dihydroxy-5α-cholan-3-one 24-oic acid (3kACA), a structurally similar bile acid released by spermiated males, but lacking the C24 sulphate ester, was tested in bioassays at concentrations between 10(-11) and 10(-14) molar (M). 3kACA did not induce upstream movement in females or additional reproductive behaviours. In contrast, spermiated male washings induced upstream movement, prolonged retention on a nest and induced an array of nesting behaviours. Differential extraction and elution by solid-phase extraction resins showed that components other than 3kPZS + 3kACA are necessary to retain females on nests and induce nest cleaning behaviours. All pheromone components, including components in addition to 3kPZS + 3kACA that retain females and induce nest cleaning behaviours were released from the anterior region of the males, as had been reported for 3kPZS. It is concluded that the sea lamprey male mating pheromone has multiple functions and is composed of multiple components.
研究了 C24 硫酸盐在交配信息素成分 7α,12α,24-三羟基-5α-胆烷-3-酮 24-硫酸盐 (3kPZS) 中对诱导产卵雌海七鳃鳗 Petromyzon marinus 上游运动的作用。7α,12α-二羟基-5α-胆烷-3-酮 24-羧酸 (3kACA) 是一种由精子释放的结构相似的胆酸，但缺乏 C24 硫酸盐酯，在 10(-11) 到 10(-14) 摩尔 (M) 的浓度下进行了生物测定。3kACA 不会诱导雌性或其他生殖行为的上游运动。相比之下，精子释放的雄性洗涤液会诱导雌性的上游运动、延长在巢上的停留时间并诱导一系列筑巢行为。固相萃取树脂的差异提取和洗脱表明，除 3kPZS + 3kACA 之外的其他成分对于在巢上保留雌性并诱导巢清洁行为是必需的。所有的信息素成分，包括除 3kPZS + 3kACA 之外的成分，都从雄性的前区域释放出来，正如 3kPZS 所报道的那样。因此，可以得出结论，海七鳃鳗雄性交配信息素有多种功能，由多种成分组成。
