Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.
Microbiol Res. 2012 Jul 25;167(7):381-7. doi: 10.1016/j.micres.2012.01.005. Epub 2012 Feb 29.
Mitomycin C treatment of Erwinia tasmaniensis strains from Australia induced prophages and the expression of bacteriocins. The bacteriocin named tasmancin inhibited E. tasmaniensis strains from South Africa and Germany. A gene cluster with a klebicin-related operon and an immunity protein was detected on plasmid pET46 from E. tasmaniensis strain Et1/99. PCR reactions using primers directed to this region produced signals for several strains originating from Australia, but not for strains isolated in South Africa and Germany. The latter isolates lacked plasmid pET46. Bacteriophages were induced from E. tasmaniensis strains Et88 and Et14/99, both isolates from South-Eastern Australia. These phages formed plaques on several other strains from this region, as well as on E. tasmaniensis strains from South Africa and Germany. Sequencing revealed similarity of phages ϕEt88 and ϕEt14, which shared the host range on E. tasmaniensis strains. Bacteriophages and tasmancin may interfere with the viability of several related E. tasmaniensis strains in the environment of carrier strains.
丝裂霉素 C 处理来自澳大利亚的欧文氏菌属 tasmaniensis 菌株诱导了噬菌体和细菌素的表达。命名为 tasmancin 的细菌素抑制了来自南非和德国的 tasmaniensis 菌株。在来自 tasmaniensis 菌株 Et1/99 的质粒 pET46 上检测到一个带有 klebicin 相关操纵子和免疫蛋白的基因簇。使用针对该区域的引物进行 PCR 反应，产生了来自澳大利亚的多个菌株的信号，但来自南非和德国的分离株没有。后者分离株缺乏质粒 pET46。从来自澳大利亚东南部的 Et88 和 Et14/99 两个 tasmaniensis 分离株中诱导出噬菌体。这些噬菌体在该地区的其他几个菌株以及南非和德国的 tasmaniensis 菌株上形成了噬菌斑。测序表明噬菌体 ϕEt88 和 ϕEt14 相似，它们在 tasmaniensis 菌株上具有相同的宿主范围。噬菌体和 tasmancin 可能会干扰载体菌株环境中几种相关 tasmaniensis 菌株的生存能力。
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