Biomolecular and Organic Electronics, Department of Physics, Chemistry, and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden.
Biochem Biophys Res Commun. 2012 Mar 23;419(4):682-6. doi: 10.1016/j.bbrc.2012.02.077. Epub 2012 Feb 20.
A method to prepare amyloid-like fibrils functionalized with magnetic nanoparticles has been developed. The amyloid-like fibrils are prepared in a two step procedure, where insulin and magnetic nanoparticles are mixed simply by grinding in the solid state, resulting in a water soluble hybrid material. When the hybrid material is heated in aqueous acid, the insulin/nanoparticle hybrid material self assembles to form amyloid-like fibrils incorporating the magnetic nanoparticles. This results in magnetically labeled amyloid-like fibrils which has been characterized by Transmission Electron Microscopy (TEM) and electron tomography. The influence of the aggregation process on proton relaxivity is investigated. The prepared materials have potential uses in a range of bio-imaging applications.
已经开发出一种将磁性纳米颗粒功能化的类淀粉样纤维的制备方法。类淀粉样纤维是通过两步程序制备的，其中胰岛素和磁性纳米颗粒通过简单地在固态下研磨混合，形成水溶性杂化材料。当将杂化材料在酸性水溶液中加热时，胰岛素/纳米颗粒杂化材料自组装形成包含磁性纳米颗粒的类淀粉样纤维。这导致形成了磁性标记的类淀粉样纤维，其已经通过透射电子显微镜（TEM）和电子断层扫描进行了表征。研究了聚集过程对质子弛豫率的影响。所制备的材料在一系列生物成像应用中具有潜在用途。
