Environmental Microbiology, Royal Institute of Technology (KTH), 106 91 Stockholm, Sweden.
Langmuir. 2012 Jun 5;28(22):8479-85. doi: 10.1021/la300599q. Epub 2012 May 24.
Magnetic iron oxide nanoparticles (MION) for protein binding and separation were obtained from water-in-oil (w/o) and oil-in-water (o/w) microemulsions. Characterization of the prepared nanoparticles have been performed by TEM, XRD, SQUID magnetometry, and BET. Microemulsion-prepared magnetic iron oxide nanoparticles (ME-MION) with sizes ranging from 2 to 10 nm were obtained. Study on the magnetic properties at 300 K shows a large increase of the magnetization ~35 emu/g for w/o-ME-MION with superparamagnetic behavior and nanoscale dimensions in comparison with o/w-ME-MION (10 emu/g) due to larger particle size and anisotropic property. Moringa oleifera coagulation protein (MOCP) bound w/o- and o/w-ME-MION showed an enhanced performance in terms of coagulation activity. A significant interaction between the magnetic nanoparticles and the protein can be described by changes in fluorescence emission spectra. Adsorbed protein from MOCP is still retaining its functionality even after binding to the nanoparticles, thus implying the extension of this technique for various applications.
水包油 (w/o) 和油包水 (o/w) 微乳液中获得了用于蛋白质结合和分离的磁性氧化铁纳米粒子 (MION)。通过 TEM、XRD、SQUID 磁强计和 BET 对制备的纳米粒子进行了表征。得到了尺寸在 2 到 10nm 之间的微乳液制备的磁性氧化铁纳米粒子 (ME-MION)。在 300K 下对磁性的研究表明，与 o/w-ME-MION（10 emu/g）相比，具有超顺磁性和纳米尺寸的 w/o-ME-MION 的磁化强度显着增加~35 emu/g，这是由于较大的粒径和各向异性。与 w/o- 和 o/w-ME-MION 结合的辣木胶凝结蛋白 (MOCP) 表现出增强的凝结活性。通过荧光发射光谱的变化可以描述磁性纳米粒子和蛋白质之间的显著相互作用。即使结合到纳米粒子上，从 MOCP 吸附的蛋白质仍保留其功能，因此暗示该技术可扩展到各种应用。
