Gambihler S, Delius M, Brendel W
Institute for Surgical Research, University of Munich, Federal Republic of Germany.
Ultrasound Med Biol. 1990;16(6):587-94. doi: 10.1016/0301-5629(90)90024-7.
L1210 cells were exposed in suspension to shock waves generated with a Dornier XL1 lithotripter. After 1000 discharges at 25 kV, the number of nondisrupted cells was 15% and the number of trypan blue excluding cells was 7% as compared to 100% in sham treated controls; the shock-wave effect was more prominent at higher voltages and less prominent at higher discharge numbers when compared at similar electrical input energies. Overall proliferation of cells which were trypan blue negative after exposure exceeded 70% of the proliferation of sham treated controls, except after 1000 shocks at 25 kV, where proliferation was reduced to 42%. The latter reduction in proliferation was found to be due to a reduced growth for 24 h after exposure, with a return to normal proliferation during the following days. Limiting dilution analysis revealed that the reduced growth was mainly due to a transitory increase of the doubling time and not to a reduction of the number of proliferating cells. Cell disruption by shock waves was completely inhibited by exposing the cells at an elevated pressure of 101 atmospheres, pointing to the possible involvement of cavitation in the shock wave effect.
将L1210细胞悬浮培养，使其暴露于Dornier XL1型碎石机产生的冲击波中。在25 kV下进行1000次放电后，未受破坏的细胞数量为15%，台盼蓝拒染细胞数量为7%，而假处理对照组为100%；在相似的电输入能量下比较，冲击波效应在较高电压时更显著，在较高放电次数时则不那么显著。暴露后台盼蓝阴性的细胞总体增殖超过假处理对照组增殖的70%，但在25 kV下进行1000次冲击后除外，此时增殖降至42%。发现后者增殖减少是由于暴露后24小时内生长减缓，随后几天恢复正常增殖。极限稀释分析表明，生长减缓主要是由于倍增时间短暂增加，而非增殖细胞数量减少。通过在101个大气压的高压下暴露细胞，可完全抑制冲击波对细胞的破坏，这表明空化作用可能参与了冲击波效应。
