Central Lab, Chaozhou Central Hospital, Chaozhou, Guangdong Province, China.
PLoS One. 2012;7(2):e32149. doi: 10.1371/journal.pone.0032149. Epub 2012 Feb 24.
Human papilloma virus (HPV) infection was the main cause of cervical cancer. There were only a few reports and detailed data about epidemiological research of HPV infection in rural population of China.
The cervical cells of rural Chaozhou women were collected, and multiplex real time PCR was firstly performed to detect high-risk HPV (HR-HPV) infection, which could detect 13 types of HR-HPV (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68). Then, HPV-positive samples were typed by HPV GenoArray test.
HR-HPV DNA was detected by multiplex real time-PCR in 3830 of 48559 cases (7.89%). There was a peak incidence in age of 55-60 years group, and a lower incidence in who lived in plain group compared with suburban, mountain and seashore group. 3380 cases of HPV positive sample were genotyped, 11.01% (372/3380) cases could not be classified, among the typed 3008 cases, 101 cases were identified without HR-HPV type infection, 2907 cases were infected with one HR-HPV type at least, the 6 most common HR-HPV types in descending order of infection, were type 52 (33.4%, 16 (20.95%), 58 (15.93%), 33 (9.94%), 68 (9.22%) and 18 (8.36%). The combined prevalence of HPV types 16 and 18 accounted for 28.52% of total infection. However, type 52 plus 58 presented 48.23% of total infection. 2209/2907 cases were infected with a single HPV type and 698/2907 cases were infected with multiple types, and multiple infection constituent ratio increased with age, with a peak incidence in age 55-60 years group.
Our findings showed low prevalence of HPV vaccine types (16 and 18) and relatively high prevalence of HPV-52 and -58, support the hypothesis that the second-generation HPV vaccines including HPV-52 and -58 may offer higher protection for women in rural Guangdong Province.
人乳头瘤病毒（HPV）感染是宫颈癌的主要病因。目前，中国农村人群 HPV 感染的流行病学研究仅有少数报道和详细数据。
采集潮汕农村地区妇女的宫颈细胞，首先采用多重实时 PCR 法检测高危型 HPV（HR-HPV）感染，该法可检测 13 种 HR-HPV（16、18、31、33、35、39、45、51、52、56、58、59 和 68）。然后，采用 HPV GenoArray 试验对 HPV 阳性样本进行分型。
3830/48559 例（7.89%）患者经多重实时 PCR 法检测到 HR-HPV DNA。高发年龄组为 55-60 岁组，平原地区组的发病率低于郊区、山区和沿海地区组。对 3380 例 HPV 阳性样本进行基因分型，11.01%（372/3380）的样本无法分类，在已分型的 3008 例中，有 101 例未检出 HR-HPV 型感染，2907 例至少感染 1 种 HR-HPV 型，感染率由高到低依次为 HPV 52 型（33.4%）、16 型（20.95%）、58 型（15.93%）、33 型（9.94%）、68 型（9.22%）和 18 型（8.36%）。HPV 16 和 18 型联合流行率占总感染率的 28.52%，而 HPV 52 加 58 型的流行率占总感染率的 48.23%。2209/2907 例患者感染单一 HPV 型，698/2907 例患者感染多种 HPV 型，且随年龄增长，多重感染构成比增加，高发年龄组为 55-60 岁组。
本研究发现 HPV 疫苗型（16 和 18）的流行率较低，而 HPV-52 和 -58 的流行率相对较高，支持第二代 HPV 疫苗（包括 HPV-52 和 -58）可能为广东农村地区女性提供更高保护的假说。
