Biology Department, Harvey Mudd College, Claremont, California 91711.
G3 (Bethesda). 2011 Dec;1(7):603-6. doi: 10.1534/g3.111.001404. Epub 2011 Dec 1.
The molecular control of gene expression in development is mediated through the activity of embryonic enhancer cis-regulatory modules. This activity is determined by the combination of repressor and activator transcription factors that bind at specific DNA sequences in the enhancer. A proposed mechanism to ensure a high fidelity of transcriptional output is functional redundancy between closely spaced binding sites within an enhancer. Here I show that at the bithorax complex in Drosophila there is selective redundancy for both repressor and activator factor binding sites in vivo. The absence of compensatory binding sites is responsible for two rare gain-of-function mutations in the complex.
胚胎增强子顺式调控模块的活性介导了发育过程中基因表达的分子调控。这种活性是由结合在增强子特定 DNA 序列上的抑制因子和激活转录因子的组合决定的。一个确保转录输出高保真度的建议机制是增强子内紧密间隔的结合位点之间的功能冗余。在这里，我表明在果蝇的双胸复合体中，体内存在针对抑制因子和激活因子结合位点的选择性冗余。缺乏补偿结合位点是该复合体中两个罕见的功能获得性突变的原因。
