Anal Chem. 2012 Apr 3;84(7):3049-52. doi: 10.1021/ac203455b. Epub 2012 Mar 12.
We report a new type of microarray, based on glyconanoparticles (GNPs), to study glycan-lectin interactions. GNPs, synthesized by conjugating carbohydrate ligands on silica nanoparticles, were printed on a photoactive surface followed by covalent immobilization by light activation. The GNP microarrays could be probed by lectins labeled with fluorescein as well as fluorescein-doped silica nanoparticles (FSNPs). Results showed that FSNP as the label enhanced the signals for the higher affinity ligands than the lower ones.
我们报告了一种基于糖纳米粒子(GNPs)的新型微阵列,用于研究糖-凝集素相互作用。GNPs 通过在硅纳米粒子上缀合碳水化合物配体合成,然后通过光激活进行共价固定而被打印在光活性表面上。GNPs 微阵列可以通过用荧光素标记的凝集素以及荧光素掺杂的硅纳米粒子(FSNPs)探测。结果表明,与低亲和力配体相比,FSNP 作为标记物增强了对高亲和力配体的信号。