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[食品中沙门氏菌的检测方法]

[Methods for the detection of salmonellae in food].

作者信息

Paunović M

机构信息

Zavod za preventivnu medicinu, Vojnomedicinska akademija.

出版信息

Vojnosanit Pregl. 1990 Jul-Aug;47(4):280-5.

PMID:2238513
Abstract

Presented are results of modern immunological and culture medium techniques for the salmonella isolation from chicken trunks compared with those obtained by standard technique (ST). By using fluorescent antibody technique (FAT), enriched serum techniques (ES) and immune stain-reaction (ISR) a larger number of positive samples was detected (31%-41%) in comparison with standard culture medium techniques (ST) (25%). Statistically significant differences were obtained by FAT. By both Rappaport-Vassiliadis (RV) and modified semi-solid Rappaport-Vassiliadis (MSRV) culture medium techniques about thrice higher percentage of salmonella positive findings were obtained than in comparison with the standard techniques. Compared to RV, the MSRV culture media technique reduces the salmonella isolation period for 48.

摘要

本文展示了用于从鸡躯干中分离沙门氏菌的现代免疫学和培养基技术的结果,并与标准技术(ST)所获结果进行了比较。通过使用荧光抗体技术(FAT)、富集血清技术(ES)和免疫染色反应(ISR),与标准培养基技术(ST,25%)相比,检测到更多的阳性样本(31%-41%)。通过FAT获得了具有统计学意义的差异。通过Rappaport-Vassiliadis(RV)培养基技术和改良的半固体Rappaport-Vassiliadis(MSRV)培养基技术获得的沙门氏菌阳性检出率比标准技术高出约三倍。与RV相比,MSRV培养基技术将沙门氏菌分离时间缩短了48小时。

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