Mercado E C
Departamento de Biología, Instituto Nacional de Farmacología y Bromatología, Buenos Aires, Argentina.
Rev Argent Microbiol. 1983;15(4):187-92.
Thirty-five samples of eviscerated poultry from processing plants in greater Buenos Aires were examined for the presence of Salmonella. Eighteen samples (51.2%) were found to be contaminated with salmonellae on testing with the non-destructive rinse method. Out of 7 plants investigated, 6 exhibited the presence of Salmonella in the product. A total of 6 confirmed species were found, S. infantis being the most frequently isolated. Rappaport-Vassiliadis and Tetrathionate-Brilliant Green broths were used as enrichment media, combined with Bismuth Sulphite or Brilliant Green-Phenol Red-Lactose-Sucrose-Deoxycholate agars as isolations media. The combination Rappaport-Vassiliadis broth and Bismuth Sulphite agar, was the only one that allowed the detection of all positive samples, including the six confirmed Salmonella species. Taking in to account the percentage of presumed colonies that were later confirmed, the Rappaport-Vassiliadis broth/Bismuth Sulphite agar proved the most specific.
对来自大布宜诺斯艾利斯地区加工厂的35份去内脏家禽样本进行了沙门氏菌检测。采用无损冲洗法检测时,18份样本(51.2%)被发现受到沙门氏菌污染。在调查的7家工厂中,有6家产品中检测出沙门氏菌。共发现6种已确认的菌种,其中婴儿沙门氏菌是最常分离出的菌种。使用Rappaport-Vassiliadis肉汤和四硫磺酸盐-煌绿肉汤作为增菌培养基,结合亚硫酸铋或煌绿-酚红-乳糖-蔗糖-脱氧胆酸盐琼脂作为分离培养基。Rappaport-Vassiliadis肉汤和亚硫酸铋琼脂的组合是唯一能检测出所有阳性样本的组合,包括6种已确认的沙门氏菌菌种。考虑到后来被确认为阳性的假定菌落的百分比,Rappaport-Vassiliadis肉汤/亚硫酸铋琼脂被证明是最具特异性的。