González-García Estefanía, Marina María Luisa, García María Concepción, Righetti Pier Giorgio, Fasoli Elisa
Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares Madrid, Spain.
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 20131 Milan, Italy.
J Proteomics. 2016 Oct 4;148:105-12. doi: 10.1016/j.jprot.2016.07.024. Epub 2016 Jul 25.
Plum (Prunus domestica L.) and peach (Prunus persica (L.) Batsch) seed proteins are a source of bioactive peptides. These seeds, though, are usual residues produced during canning and beverage preparation that, in most cases, are irreversibly lost. The recovery and identification of these proteins might be of importance in human nutrition. This work employs the combinatorial peptide ligand libraries (CPLLs) technology as a tool to reduce the proteins dynamic concentration range. The most suitable extraction and CPLL capture conditions have been obtained and applied for the comprehensive identification of seed proteins. The analysis of recovered species by nLC-MS/MS has allowed the identification of 141 and 97 unique gene products from plum and peach seeds, respectively. It was possible to identify 16 proteins belonging to the Prunus genus. Moreover, a high number of histones and seed storage proteins were identified. Additionally, 21 and 14 bioactive peptides previously identified were found within protein sequences in plum and peach seeds, respectively.
Plums and peaches seeds are cheap sources of proteins that are irretrievably lost after canning and beverage production. Although this kind of residues has been used in animal feed or production of biofuel, they are usually incinerated or sent to landfills, wasting their huge potential. In order to exploit this, it is important to comprehensively study proteins present in plum and peach seeds. Nevertheless, since proteomics analysis is in most cases handicapped by the presence of high-abundance proteins masking the detection of the low-abundance ones, it is important to overcome this challenge. In this sense, combinatorial peptide ligand libraries (CPLLs) have been used in this work to reduce the dynamic protein concentration range to enable the identification of a higher amount of proteins than employing conventional methods. In this work, the better extracting conditions have been optimized and up to 141 and 97 unique gene products from plum and peach seeds have been found, respectively. Moreover, 21 and 14 peptides previously identified as bioactive peptides were ascertained within protein sequences in plum and peach seeds, respectively. For that reason, this research takes the first step in the recovery of these valuable proteins and in the extraction of bioactive peptides, which could be successfully adopted in human nutrition.
李子（欧洲李）和桃子（桃）种子蛋白是生物活性肽的来源。然而，这些种子通常是罐头加工和饮料制备过程中产生的残渣，在大多数情况下会不可逆转地流失。这些蛋白质的回收和鉴定对人类营养可能具有重要意义。本研究采用组合肽配体文库（CPLL）技术作为工具来缩小蛋白质的动态浓度范围。已获得最合适的提取和CPLL捕获条件，并将其应用于种子蛋白的全面鉴定。通过nLC-MS/MS对回收物种的分析分别鉴定出李子和桃子种子中的141种和97种独特基因产物。有可能鉴定出16种属于李属的蛋白质。此外，还鉴定出大量组蛋白和种子贮藏蛋白。此外，分别在李子和桃子种子的蛋白质序列中发现了先前鉴定的21种和14种生物活性肽。
李子和桃子种子是廉价的蛋白质来源，在罐头加工和饮料生产后会无可挽回地流失。虽然这类残渣已用于动物饲料或生物燃料生产，但它们通常被焚烧或送往垃圾填埋场，浪费了其巨大潜力。为了利用这一点，全面研究李子和桃子种子中的蛋白质很重要。然而，由于蛋白质组学分析在大多数情况下受到高丰度蛋白质掩盖低丰度蛋白质检测的阻碍，克服这一挑战很重要。从这个意义上说，本研究使用组合肽配体文库（CPLL）来缩小蛋白质的动态浓度范围，以便能够比采用传统方法鉴定更多的蛋白质。在本研究中，优化了更好的提取条件，分别从李子和桃子种子中发现了多达141种和97种独特基因产物。此外，分别在李子和桃子种子的蛋白质序列中确定了先前鉴定为生物活性肽的21种和14种肽。因此，本研究在回收这些有价值的蛋白质和提取生物活性肽方面迈出了第一步，这可以成功应用于人类营养。
