重要的是，枝孢菌属（Micromonospora spp.）作为淡水湖纤维素的定殖者，通过定量逆转录聚合酶链反应（qRT-PCR）的 16S rRNA 得到证实。
Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA.
机构信息
Microbiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom.
出版信息
Appl Environ Microbiol. 2012 May;78(9):3495-9. doi: 10.1128/AEM.07314-11. Epub 2012 Mar 2.
Abstract
The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.
摘要
采用定量 PCR（qPCR）对反转录的 16S rRNA 进行分析，测定了栖息在纤维素诱饵、水柱和两个淡水湖沉积物中的细菌群落中的小单孢菌相对丰度。小单孢菌是在湖底纤维素基质上定植的活性细菌种群的重要成员，通过 CFU 计数证实了它们在更深的深度时更为普遍。
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