Zhou Wen, Håkansson Kristina
Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.
Curr Proteomics. 2011 Dec 1;8(4):297-308. doi: 10.2174/157016411798220826.
Fourier transform tandem mass spectrometry (MS/MS) provides high mass accuracy, high sensitivity, and analytical versatility and has therefore emerged as an indispensable tool for structural elucidation of biomolecules. Glycosylation is one of the most common posttranslational modifications, occurring in ~50% of proteins. However, due to the structural diversity of carbohydrates, arising from non-template driven biosynthesis, achievement of detailed structural insight is highly challenging. This review briefly discusses carbohydrate sample preparation and ionization methods, and highlights recent developments in alternative high-resolution MS/MS strategies, including infrared multiphoton dissociation (IRMPD), electron capture dissociation (ECD), and electron detachment dissociation (EDD), for carbohydrates with a focus on glycans and proteoglycans from mammalian glycoproteins.
傅里叶变换串联质谱（MS/MS）具有高质量准确性、高灵敏度和分析多功能性，因此已成为生物分子结构解析中不可或缺的工具。糖基化是最常见的翻译后修饰之一，约50%的蛋白质中都会发生。然而，由于非模板驱动的生物合成导致碳水化合物结构多样，要实现对其详细结构的深入了解极具挑战性。本文简要讨论了碳水化合物样品制备和电离方法，并重点介绍了替代高分辨率MS/MS策略的最新进展，包括红外多光子解离（IRMPD）、电子捕获解离（ECD）和电子脱附解离（EDD），主要针对来自哺乳动物糖蛋白的聚糖和蛋白聚糖等碳水化合物。
