Ibarra-Trujillo Consuelo, Villar-Vidal María, Gaitán-Cepeda Luis Alberto, Pozos-Guillen Amaury, Mendoza-de Elias Roberto, Sánchez-Vargas Luis Octavio
Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
Rev Iberoam Micol. 2012 Oct-Dec;29(4):214-22. doi: 10.1016/j.riam.2012.02.003. Epub 2012 Mar 3.
This study quantifies the production of single and mixed biofilms of Candida albicans and Staphylococcus aureus to determine if such mixed biofilms have synergistic effects. Assays were performed using polystyrene microtitre plates of 96 wells, metabolic activity was measured by the enzymatic reduction of a tetrazolium salt (XTT) and colorimetric changes were measured at 490 nm. Confocal scanning laser microscopy was used to visualise the biofilms of each microorganism and its growth kinetics. The highest levels of biofilm formation were observed in mixed biofilms, followed by those of Candida albicans only, with the lowest levels of biofilm formation being detected for Staphylococcus aureus; all together these results suggest a synergistic relationship between the tested microorganisms.
本研究对白色念珠菌和金黄色葡萄球菌的单一生物膜及混合生物膜的形成进行了量化，以确定此类混合生物膜是否具有协同效应。实验采用96孔聚苯乙烯微量滴定板进行，通过四唑盐（XTT）的酶促还原反应来测定代谢活性，并在490nm波长下测量比色变化。利用共聚焦扫描激光显微镜观察每种微生物的生物膜及其生长动力学。混合生物膜中观察到的生物膜形成水平最高，其次是仅白色念珠菌的生物膜，金黄色葡萄球菌的生物膜形成水平最低；所有这些结果表明，受试微生物之间存在协同关系。
