Touil Hidaya F Z, Boucherit Kebir, Boucherit-Otmani Zahia, Khoder Ghalia, Madkour Mohamed, Soliman Sameh S M
Laboratory Antibiotics Antifungals: Physico-Chemical, Synthesis and Biological Activity (LapSab), Tlemcen University, Tlemcen B.P 119, Algeria.
University Center Belhadj Bouchaïb, Aïn Temouchent BP 284, Algeria.
Biomolecules. 2020 Feb 21;10(2):342. doi: 10.3390/biom10020342.
is one of the most common human fungal pathogens and represents the most important cause of opportunistic mycoses worldwide. Surgical devices including catheters are easily contaminated with via its formation of drug-resistant biofilms. In this study, amphotericin-B-resistant strains were isolated from surgical devices at an intensive care center. The objective of this study was to develop optimized effective inhibitory treatment of resistant by terpenoids, known to be produced naturally as protective signals. Endogenously produced farnesol by yeast and plant terpenoids, carvacrol, and cuminaldehyde were tested separately or in combination on amphotericin-B-resistant in either single- or mixed-infections. The results showed that farnesol did not inhibit hyphae formation when associated with bacteria. Carvacrol and cuminaldehyde showed variable inhibitory effects on yeast compared to hyphae formation. A combination of farnesol with carvacrol showed synergistic inhibitory activities not only on yeast and hyphae, but also on biofilms formed from single- and mixed-species and at reduced doses. The combined terpenoids also showed biofilm-penetration capability. The aforementioned terpenoid combination will not only be useful in the treatment of different resistant forms, but also in the safe prevention of biofilm formation.
是最常见的人类真菌病原体之一，也是全球机会性真菌病的最重要病因。包括导管在内的外科手术器械很容易通过形成耐药生物膜而被其污染。在本研究中，从一家重症监护中心的外科手术器械中分离出了对两性霉素B耐药的菌株。本研究的目的是开发针对耐药菌的优化有效抑制治疗方法，已知萜类化合物可作为天然保护信号产生。分别或联合测试了由酵母内源性产生的法尼醇以及植物萜类化合物香芹酚和枯茗醛对单感染或混合感染的两性霉素B耐药菌的作用。结果表明，法尼醇与细菌共存时不抑制菌丝形成。与菌丝形成相比，香芹酚和枯茗醛对酵母显示出不同的抑制作用。法尼醇与香芹酚的组合不仅对酵母和菌丝有协同抑制活性，而且对单物种和混合物种形成的生物膜也有协同抑制活性，且剂量降低。联合使用的萜类化合物还显示出生物膜穿透能力。上述萜类化合物组合不仅可用于治疗不同的耐药菌形式，还可用于安全预防生物膜形成。
