Nakamura S, Kawamata T, Akiguchi I, Kameyama M, Nakamura N, Kimura H
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Acta Neuropathol. 1990;80(4):419-25. doi: 10.1007/BF00307697.
Monoamine oxidase (MAO) histochemistry has been performed in brains from patients with dementia of Alzheimer type (DAT) and aged controls. Conspicuous MAO-positive cell clusters were frequently observed in the amygdala, hippocampus, and insular cortex in the brains of DAT. Double staining with glial fibrillary acidic protein immunohistochemistry revealed that the cluster-forming MAO-positive cells were astrocytes. Using Bielschowsky's method, Congo red and thioflavin S counterstaining, this astrocytic mass was shown to be associated with senile plaques. By the enzyme inhibition experiment, MAO activity in senile plaques was revealed to be of type B. The present results clearly indicate that MAO-B activity is expressed in fibrillary astrocytes in or around senile plaques, suggesting that these astrocytes metabolize exogenous amines in senile plaques.
已对阿尔茨海默型痴呆（DAT）患者和老年对照者的大脑进行了单胺氧化酶（MAO）组织化学研究。在DAT患者大脑的杏仁核、海马体和岛叶皮质中经常观察到明显的MAO阳性细胞簇。用胶质纤维酸性蛋白免疫组织化学进行双重染色显示，形成簇的MAO阳性细胞是星形胶质细胞。使用 Bielschowsky 方法、刚果红和硫黄素 S 复染，结果表明这种星形胶质细胞团与老年斑有关。通过酶抑制实验，发现老年斑中的MAO活性为B型。目前的结果清楚地表明，MAO-B活性在老年斑内或其周围的纤维性星形胶质细胞中表达，提示这些星形胶质细胞代谢老年斑中的外源性胺类。
