电刺激后及恢复过程中人类骨骼肌单根肌纤维中的ATP含量。

ATP content in single fibres from human skeletal muscle after electrical stimulation and during recovery.

作者信息

Söderlund K, Hultman E

机构信息

Department of Clinical Chemistry II, Huddinge University Hospital, Karolinska Institutet, Sweden.

出版信息

Acta Physiol Scand. 1990 Jul;139(3):459-66. doi: 10.1111/j.1748-1716.1990.tb08947.x.

DOI:10.1111/j.1748-1716.1990.tb08947.x

PMID:2239349

Abstract

The ATP content was measured in type I and type II fibres from human vastus lateralis muscle at rest, after electrical stimulation and during recovery. At rest the mean values were 25.2 +/- 4.02 and 25.9 +/- 3.62 mmol kg-1 dry muscle (mean +/- SD) for type I and type II fibres respectively. Normal distribution curves were found for both types I and II fibres. After intermittent electrical stimulation for 83 s (1.6 s stimulation, 1.6 s pause) with occluded blood flow, the force generation decreased to 22% of the initial value and the muscle tissue showed a mean decrease in ATP to 14.8 and in phosphocreatine to 5.44 mmol kg-1 dry muscle; lactate increased to 128.9 mmol kg-1 dry muscle. The ATP content in isolated fibres was equally decreased in both fibre types to 16 mmol kg-1 dry muscle. In 11% of the fibres the ATP content was lower than 10 mmol kg-1 dry muscle. After 15 min rest with intact blood circulation ATP was completely resynthesized in type I fibres and to 91% in type II fibres.

摘要

在静息状态、电刺激后及恢复过程中，对取自人体股外侧肌的I型和II型纤维中的三磷酸腺苷（ATP）含量进行了测定。静息时，I型和II型纤维中ATP的平均值分别为25.2±4.02和25.9±3.62 mmol·kg⁻¹干肌（平均值±标准差）。I型和II型纤维均呈现正态分布曲线。在血流阻断的情况下进行83秒的间歇性电刺激（1.6秒刺激，1.6秒间歇）后，肌力生成降至初始值的22%，肌肉组织中ATP平均降至14.8 mmol·kg⁻¹干肌，磷酸肌酸降至5.44 mmol·kg⁻¹干肌；乳酸增加至128.9 mmol·kg⁻¹干肌。两种纤维类型中分离纤维的ATP含量均同等下降至16 mmol·kg⁻¹干肌。11%的纤维中ATP含量低于10 mmol·kg⁻¹干肌。在血液循环完整的情况下休息15分钟后，I型纤维中的ATP完全重新合成，II型纤维中的ATP重新合成至91%。