Yang Dian, Liu Chao, Liu Hong
Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China.
Fa Yi Xue Za Zhi. 2011 Dec;27(6):441-3.
To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues.
DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with Quantifiler kit, followed by amplification with Sinofiler kit or Minifiler kit.
DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/microL. Sixty-two samples whose DNA concentration were more than 0.020 ng/microL had obtained 9 or more STR loci successfully. The detection rate was 68.13%.
The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.
