Interdisciplinary Nanoscience Center (iNANO), Departments of Molecular Biology, Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark.
Nanoscale. 2012 Apr 7;4(7):2352-61. doi: 10.1039/c2nr11554a. Epub 2012 Mar 6.
Iron oxide nanoparticles have found widespread applications in different areas including cell separation, drug delivery and as contrast agents. Due to water insolubility and stability issues, nanoparticles utilized for biological applications require coatings such as the commonly employed polyethylene glycol (PEG). Despite its frequent use, the influence of PEG coatings on the physicochemical and biological properties of iron nanoparticles has hitherto not been studied in detail. To address this, we studied the effect of 333-20,000 Da PEG coatings that resulted in larger hydrodynamic size, lower surface charge, longer circulation half-life, and lower uptake in macrophage cells when the particles were coated with high molecular weight (M(w)) PEG molecules. By use of magnetic resonance imaging, we show coating-dependent in vivo uptake in murine tumors with an optimal coating M(w) of 10,000 Da.
氧化铁纳米粒子在细胞分离、药物输送和作为对比剂等不同领域得到了广泛的应用。由于水不溶性和稳定性问题，用于生物应用的纳米粒子需要涂层，如常用的聚乙二醇（PEG）。尽管它被频繁使用，但 PEG 涂层对铁纳米粒子的物理化学和生物特性的影响迄今尚未详细研究。为了解决这个问题，我们研究了 333-20,000 Da 的 PEG 涂层的影响，结果表明，当使用高分子量（M（w））PEG 分子对颗粒进行涂层时，颗粒的水动力尺寸更大，表面电荷更低，循环半衰期更长，在巨噬细胞中的摄取量更低。通过磁共振成像，我们显示了与涂层相关的体内摄取在小鼠肿瘤中，最佳的涂层 M（w）为 10,000 Da。
