视网膜血氧计图像必须标准化：方法学分析。

Retinal oximetry images must be standardized: a methodological analysis.

机构信息

University of Iceland, National University Hospital, Reykjavik, Iceland.

出版信息

Invest Ophthalmol Vis Sci. 2012 Apr 2;53(4):1729-33. doi: 10.1167/iovs.11-8621.

DOI:10.1167/iovs.11-8621

PMID:22395877

Abstract

PURPOSE

Retinal vessel oximetry is a new technology and needs detailed methodological scrutiny. We determine (1) the repeatability of retinal vessel oxygen saturation measurements, (2) whether measured saturation is different between retinal quadrants, and (3) whether the angle of gaze changes measurements of the same vessels.

METHODS

Fundus oximetry images were obtained from 26 healthy individuals, 18 to 30 years old, using the Oxymap retinal oximeter. Oxygen saturation in the same vessel segments was compared between two similar images of each individual to determine repeatability. Vessel oxygen saturation was also compared between different quadrants of the retina in the same oximetry image. Finally, oxygen saturation measurements were made on the same vessel segments at different angles of gaze.

RESULTS

Mean and standard deviation of saturation measurements was 93.1% ± 2.3% in arterioles and 64.9% ± 3.3% in venules. Standard deviation of repeated saturation measurements on the same vessel segment was 1.0% in arterioles and 1.4% in venules. Significant differences were seen between retinal quadrants. When angle of gaze was altered, measured saturation was lower in the same vessels when they were located in the inferior portion compared with other parts of the image (-1.3% ± 1.7%, P = 0.0004 in arterioles and -1.9 ± 2.4%, P = 0.0007 in venules).

CONCLUSIONS

Retinal vessel oxygen saturation measurements are repeatable with a small standard deviation. When oximetry results are compared between time points or eyes, the imaging must be standardized and similar parts of the images analyzed.

摘要

目的

视网膜血管血氧测定是一项新技术，需要对其进行详细的方法学研究。我们旨在：（1）确定视网膜血管血氧饱和度测量的可重复性；（2）测量的饱和度是否在视网膜象限之间存在差异；（3）注视角度的变化是否会影响同一血管的测量。

方法

使用 Oxymap 视网膜血氧仪对 26 名 18 至 30 岁的健康个体进行眼底血氧测量。通过比较每个个体的两个相似眼底图像中相同血管段的氧饱和度，确定重复性。还比较了同一眼底图像中不同象限的血管氧饱和度。最后，在不同的注视角度下对同一血管段进行氧饱和度测量。

结果

动脉的平均饱和度及其标准差为 93.1%±2.3%，静脉为 64.9%±3.3%。同一血管段重复测量的饱和度标准差为动脉的 1.0%，静脉的 1.4%。视网膜象限之间存在显著差异。当改变注视角度时，同一血管在位于下方部分时的测量饱和度低于其他部位（动脉为-1.3%±1.7%，P=0.0004；静脉为-1.9±2.4%，P=0.0007）。