Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Phytochem Anal. 2012 Sep-Oct;23(5):554-8. doi: 10.1002/pca.2356. Epub 2012 Mar 6.
Stemona spp. have been traditionally used as natural pesticides and medicinal plants. Stemona collinsiae Craib has been of interest for its insecticidal activity, which has been supported by many scientific research studies. The roots contain didehydrostemofoline and stemofoline alkaloids as active components.
To develop and validate a HPLC method for the quantitative analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts.
HPLC was carried out using a Hypersil BDS C₁₈-column eluted with methanol:1 m m ammonium acetate (55:45) with a flow rate of 1 mL/min and detection at 295 nm. Method validation was performed to assure its linearity, precision, accuracy and limits of detection and quantitation.
Didehydrostemofoline and stemofoline showed a linear relationship within the range of 0.5-432.4 and 0.5-188.4 µg/mL, respectively. The method was shown to be precise with RSD < 2%. The average recovery of didehydrostemofoline and stemofoline were 98.80 and 99.97%, respectively. Eight samples of S. collinsiae root extracts were analysed and the average contents of didehydrostemofoline and stemofoline were 0.78 and 0.048% w/w, respectively.
The HPLC method developed was appropriate for the analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts. This work would be useful as a guide for the standardisation of S. collinsiae root extract raw materials and their products as natural pesticides.
百部属植物一直被传统用作天然杀虫剂和药用植物。百部（Stemona collinsiae）因其杀虫活性而受到关注，许多科学研究都支持这一点。其根部含有二氢对叶百部碱和百部碱作为活性成分。
建立并验证一种高效液相色谱法，用于定量分析百部（Stemona collinsiae）根提取物中的二氢对叶百部碱和百部碱。
采用 Hypersil BDS C₁₈ 柱，以甲醇：1 m m 乙酸铵（55:45）为流动相，流速为 1 mL/min，检测波长为 295nm，进行 HPLC 分析。对方法进行验证，以确保其线性、精密度、准确度和检测限及定量限。
二氢对叶百部碱和百部碱的线性范围分别为 0.5-432.4 和 0.5-188.4μg/mL。该方法精密度良好，RSD<2%。二氢对叶百部碱和百部碱的平均回收率分别为 98.80%和 99.97%。对 8 个百部（Stemona collinsiae）根提取物样品进行分析，二氢对叶百部碱和百部碱的平均含量分别为 0.78%和 0.048%（w/w）。
所建立的 HPLC 方法适用于百部（Stemona collinsiae）根提取物中二氢对叶百部碱和百部碱的分析。本研究为百部（Stemona collinsiae）根提取物及其作为天然杀虫剂的产品的标准化提供了参考。
