采用高效液相色谱-二极管阵列检测-质谱法测定植物材料中环状鞣花单宁oenothein B：方法开发与验证。
Determination of macrocyclic ellagitannin oenothein B in plant materials by HPLC-DAD-MS: method development and validation.
机构信息
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Poland.
出版信息
Phytochem Anal. 2012 Nov-Dec;23(6):582-7. doi: 10.1002/pca.2358. Epub 2012 Mar 6.
INTRODUCTION
Oenothein B (OeB) is a dimeric macrocyclic ellagitannin occurring mainly in the genus Oenothera. In the literature no validated HPLC method for quantification of this compound has been reported.
OBJECTIVE
To develop and validate an efficient HPLC method for quantification of OeB in plant materials.
METHODOLOGY
Extraction conditions of OeB from plant material were optimised. Then the first validated HPLC method for quantification was developed.
RESULTS
We have shown that ultrasonic-assisted extraction at 40 °C in 15 min using water are the most optimal conditions for OeB extraction. We have quantified OeB in several plant materials belonging to Oenotheraceae family. The lowest amount of OeB was present in Circaea lutetiana herb (27.64 ± 0.26 mg/g) and the highest was quantified in Epilobium hirsutum aerial parts (72.91 ± 1.38 mg/g).
CONCLUSION
The first HPLC method for quantification of OeB in plant materials was developed and validated. We also for the first time optimised the extraction conditions for OeB.
简介
奥替壬素 B（OeB）是一种主要存在于月见草属的二聚大环鞣花单宁。在文献中，尚未报道用于定量此化合物的经过验证的 HPLC 方法。
目的
开发和验证一种用于植物材料中 OeB 定量的高效 HPLC 方法。
方法
优化了从植物材料中提取 OeB 的条件。然后开发了第一个经过验证的 HPLC 定量方法。
结果
我们表明，在 40°C 下使用水进行 15 分钟的超声波辅助提取是提取 OeB 的最佳条件。我们已经定量了几种属于柳叶菜科的植物材料中的 OeB。OeB 的含量最低的是柳叶菜属植物（27.64±0.26mg/g），含量最高的是黄花补血草地上部分（72.91±1.38mg/g）。
结论
开发并验证了用于植物材料中 OeB 定量的第一个 HPLC 方法。我们还首次优化了 OeB 的提取条件。
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