通过电子顺磁共振波谱研究人类端粒四链体的细胞内构象。
Intracellular conformations of human telomeric quadruplexes studied by electron paramagnetic resonance spectroscopy.
机构信息
Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, Konstanz, Germany.
出版信息
Chemphyschem. 2012 Apr 23;13(6):1444-7. doi: 10.1002/cphc.201100980. Epub 2012 Mar 6.
In-cell DEER (in-cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG)(3)]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time-dependent distance measurements are carried out to monitor quadruplex folding.
在细胞内双电子电子共振（in-cell double electron electron resonance，in-cell DEER）被应用于研究人类端粒重复 d[AGGG(TTAGGG)(3)]的四链体形成。该初始未折叠的 DNA 序列在注入活细胞后形成不同四链体拓扑结构的混合物。此外，还进行了时间依赖性距离测量以监测四链体折叠。
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