Anhui Province Key Laboratory of Forest Resource and Silviculture, Anhui Agricultural University, Hefei, 230036, China.
Plant Mol Biol. 2024 Sep 3;114(5):95. doi: 10.1007/s11103-024-01487-0.
The regulation mechanism of bamboo height growth has always been one of the hotspots in developmental biology. In the preliminary work of this project, the function of LBD transcription factor regulating height growth was firstly studied. Here, a gene PheLBD12 regulating height growth was screened. PheLBD12-overexpressing transgenic rice had shorter internodes, less bioactive gibberellic acid (GA3), and were more sensitive to GA3 than wild-type (WT) plants, which implied that PheLBD12 involve in gibberellin (GA) pathway. The transcript levels of OsGA2ox3, that encoding GAs deactivated enzyme, was significantly enhanced in PheLBD12-overexpressing transgenic rice. The transcript levels of OsAP2-39, that directly regulating the expression of EUI1 to reduce GA levels, was also significantly enhanced in PheLBD12-overexpressing transgenic rice. Expectedly, yeast one-hybrid assays, Dual-luciferase reporter assay and EMSAs suggested that PheLBD12 directly interacted with the promoter of OsGA2ox3 and OsAP2-39. Together, our results reveal that PheLBD12 regulates plant height growth by modulating GA catabolism. Through the research of this topic, it enriches the research content of LBD transcription factors and it will theoretically enrich the research content of height growth regulation.
竹子株高生长的调控机制一直是发育生物学的热点之一。在本项目的初步工作中，我们首次研究了 LBD 转录因子调控株高生长的功能。在这里，筛选到一个调控株高生长的 PheLBD12 基因。PheLBD12 过表达转基因水稻的节间更短，生物活性赤霉素（GA3）较少，对 GA3 比野生型（WT）植物更敏感，这表明 PheLBD12 参与赤霉素（GA）途径。PheLBD12 过表达转基因水稻中编码 GA 失活酶的 OsGA2ox3 的转录水平显著增强。直接调控 EUI1 表达以降低 GA 水平的 OsAP2-39 的转录水平也在 PheLBD12 过表达转基因水稻中显著增强。预期的是，酵母单杂交分析、双荧光素酶报告基因测定和 EMSA 表明 PheLBD12 直接与 OsGA2ox3 和 OsAP2-39 的启动子相互作用。总之，我们的结果表明 PheLBD12 通过调节 GA 分解代谢来调控植物株高生长。通过对这个课题的研究，丰富了 LBD 转录因子的研究内容，从理论上丰富了株高生长调控的研究内容。
