Brown A J, Roberts D C, Pritchard J E, Truswell A S
Department of Biochemistry, University of Sydney, NSW, Australia.
Am J Clin Nutr. 1990 Nov;52(5):825-33. doi: 10.1093/ajcn/52.5.825.
Twelve healthy men were fed in turn three diets for 6 wk each in a 3 x 3 randomized block design: a control diet (essentially fish free), a fish diet (200 g lean Australian fish flesh/d), and the same fish-based meal but supplemented with 5 g fish oil/d. Dietary eicosapentaenoic acid [EPA, 20: 5n-3 (omega-3)] was strongly associated with erythrocyte membrane EPA (r = 0.908 at 6 wk), strengthening its value as a measure of compliance in fish and fish-oil feeding trials. On the fish diet, subjects had increased incorporation of n-3 polyunsaturated fatty acids (PUFAs) at the expense of n-6 PUFAs in their erythrocyte membranes. When the fish-based diet was supplemented with fish oil (5 g/d), there was a significant lowering of plasma triacylglycerol (-0.16 +/- 0.24 mmol/L; mean +/- SD). No change in plasma total cholesterol was detected although the fish + oil diet produced a reduction in very-low-density-lipoprotein cholesterol (-0.24 +/- 0.26 mmol/L).
12名健康男性按照3×3随机区组设计依次接受三种饮食，每种饮食持续6周：对照饮食（基本无鱼）、鱼类饮食（每日200克澳大利亚瘦鱼肉）以及相同的以鱼为基础的膳食，但每天补充5克鱼油。膳食中的二十碳五烯酸[EPA，20:5n-3（ω-3）]与红细胞膜中的EPA密切相关（6周时r = 0.908），这增强了其作为鱼类和鱼油喂养试验中依从性衡量指标的价值。在鱼类饮食中，受试者红细胞膜中n-3多不饱和脂肪酸（PUFAs）的掺入增加，而n-6 PUFAs的掺入减少。当以鱼为基础的饮食补充鱼油（每日5克）时，血浆甘油三酯显著降低（-0.16±0.24毫摩尔/升；均值±标准差）。虽然鱼+油饮食使极低密度脂蛋白胆固醇降低（-0.24±0.26毫摩尔/升），但未检测到血浆总胆固醇有变化。
