Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan.
Nutr Metab Cardiovasc Dis. 2013 Jun;23(6):566-73. doi: 10.1016/j.numecd.2011.11.002. Epub 2012 Mar 7.
The purpose of this study was to evaluate the effects of exercise training on cardiac apoptotic pathways in obesity.
Sixteen lean Zucker rats (LZR) and sixteen obese Zucker rats (OZR) of 5-6 months of age as well as the other sixteen obese rats were subjected to treadmill running exercise for 1 h everyday for 3 months (OZR-EX). After exercise training or sedentary status of the rats, the excised hearts from the three groups were measured by heart weight index, H&E staining, TUNEL assays and Western blotting. Cardiac TUNEL-positive apoptotic cells, the protein levels of TNF alpha, Fas ligand, Fas receptors, Fas-associated death domain (FADD), Bad, Bax, activated caspase 8, activated caspase 9, and activated caspase 3 were higher in OZR than those in LZR. The protein levels of TNF alpha, Fas ligand, Fas receptors, FADD, activated caspase 8, and activated caspase 3 (Fas pathway) and the protein levels of Bad, Bax, Bax-to-Bcl2 ratio, activated caspase 9, and activated caspase 3 (mitochondria pathway) were lower in OZR-EX than those in OZR.
Cardiac Fas-dependent and mitochondria-dependent apoptotic pathways become more activated in obesity. Exercise training can prevent obesity-activated cardiac Fas-dependent and mitochondria-dependent apoptotic pathways. Our findings demonstrate a new therapeutic effect of exercise training to prevent delirious cardiac Fas-mediated and mitochondria-mediated apoptosis in obesity.
本研究旨在评估运动训练对肥胖症中心脏凋亡途径的影响。
将 16 只瘦型 Zucker 大鼠（LZR）和 16 只肥胖型 Zucker 大鼠（OZR）以及另外 16 只肥胖大鼠分为三组，分别为对照组（LZR）、肥胖对照组（OZR）和肥胖运动组（OZR-EX）。5-6 月龄时，肥胖组大鼠进行为期 3 个月的跑步机运动，每天 1 小时。运动训练或大鼠安静状态结束后，测定三组大鼠心脏重量指数、HE 染色、TUNEL 检测和 Western blot。与 LZR 相比，OZR 心脏 TUNEL 阳性凋亡细胞、TNF-α、Fas 配体、Fas 受体、Fas 相关死亡结构域（FADD）、Bad、Bax、激活的 caspase-8、激活的 caspase-9 和激活的 caspase-3 蛋白水平均较高。与 OZR 相比，OZR-EX 大鼠 TNF-α、Fas 配体、Fas 受体、FADD、激活的 caspase-8 和激活的 caspase-3（Fas 通路）以及 Bad、Bax、Bax/Bcl-2 比值、激活的 caspase-9 和激活的 caspase-3（线粒体通路）蛋白水平均较低。
肥胖可使心脏 Fas 依赖性和线粒体依赖性凋亡途径更加活跃。运动训练可预防肥胖激活的心脏 Fas 依赖性和线粒体依赖性凋亡途径。本研究结果表明，运动训练具有预防肥胖致 Fas 介导和线粒体介导的心脏凋亡的新治疗作用。
