在体脑脊液中 microRNA 的检测和定量。
In vivo microRNA detection and quantitation in cerebrospinal fluid.
机构信息
The Zucker Hillside Hospital, Glen Oaks, NY 11004, USA.
出版信息
J Mol Neurosci. 2012 Jun;47(2):243-8. doi: 10.1007/s12031-012-9731-7. Epub 2012 Mar 9.
Abstract
Alterations in microRNA (miRNA) expression in postmortem brain tissue or peripheral blood have been linked to schizophrenia. Cerebrospinal fluid might provide an in vivo biomarker more directly reflecting functional changes in the brain. The goals of this study were to determine the feasibility of detecting miRNAs in cerebrospinal fluid and to compare miRNA levels in cerebrospinal fluid versus blood. Four healthy volunteers and four patients with psychotic disorders underwent a lumbar puncture and a blood draw. Expression of 378 validated miRNAs was assessed from each biofluid type for each subject using microarray technology. Five miRNAs were chosen for validation with quantitative polymerase chain reaction. A substantial number of miRNAs (n = 95) were exclusively or predominately detected in cerebrospinal fluid (CSF). Levels of 35 miRNAs detected in both CSF and blood samples in all subjects were poorly correlated. The investigation of miRNAs in CSF can help advance the understanding of psychiatric diseases and particularly schizophrenia.
摘要
脑脊髓液中的 microRNA（miRNA）表达改变与精神分裂症有关。脑脊髓液可能提供更直接反映大脑功能变化的活体生物标志物。本研究的目的是确定检测脑脊髓液中 miRNA 的可行性，并比较脑脊髓液与血液中的 miRNA 水平。4 名健康志愿者和 4 名精神病患者接受了腰椎穿刺和血液采集。使用微阵列技术从每种生物流体类型对每位受试者的 378 种经验证的 miRNA 进行表达评估。选择 5 个 miRNA 进行定量聚合酶链反应验证。大量 miRNA（n=95）仅在脑脊髓液（CSF）中检测到或主要在脑脊髓液中检测到。在所有受试者中，在 CSF 和血液样本中检测到的 35 个 miRNA 的水平相关性较差。对 CSF 中 miRNA 的研究有助于深入了解精神疾病，特别是精神分裂症。
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本文引用的文献
1
Horizontal transfer of RNAs: exosomes as mediators of intercellular communication.RNA 的水平转移：外泌体作为细胞间通讯的介质。
Wiley Interdiscip Rev RNA. 2012 Mar-Apr;3(2):286-93. doi: 10.1002/wrna.115. Epub 2011 Oct 19.
2
Identification of microRNAs in the cerebrospinal fluid as biomarker for the diagnosis of glioma.鉴定脑脊液中的 microRNAs 作为诊断脑胶质瘤的生物标志物。
Neuro Oncol. 2012 Jan;14(1):29-33. doi: 10.1093/neuonc/nor169. Epub 2011 Sep 21.
3
Genome-wide association study identifies five new schizophrenia loci.全基因组关联研究确定了五个新的精神分裂症易感基因位点。
Nat Genet. 2011 Sep 18;43(10):969-76. doi: 10.1038/ng.940.
4
Imprinted DLK1-DIO3 region of 14q32 defines a schizophrenia-associated miRNA signature in peripheral blood mononuclear cells.14q32 印迹的 DLK1-DIO3 区域在外周血单个核细胞中定义了一个与精神分裂症相关的 miRNA 特征。
Mol Psychiatry. 2012 Jul;17(8):827-40. doi: 10.1038/mp.2011.78. Epub 2011 Jul 5.
5
Cerebrospinal fluid biomarkers for Alzheimer's disease: the present and the future.阿尔茨海默病的脑脊液生物标志物：现状与未来。
Neurodegener Dis. 2011;8(6):413-20. doi: 10.1159/000327756. Epub 2011 Jun 25.
6
Cerebrospinal fluid diagnostics in first-episode schizophrenia.首发精神分裂症的脑脊液诊断。
Eur Arch Psychiatry Clin Neurosci. 2011 Oct;261(7):529-30. doi: 10.1007/s00406-011-0193-7. Epub 2011 Feb 6.
7
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Blood. 2011 Mar 17;117(11):3140-6. doi: 10.1182/blood-2010-09-308684. Epub 2011 Jan 3.
8
Altered microRNA expression profiles in postmortem brain samples from individuals with schizophrenia and bipolar disorder.精神分裂症和双相情感障碍患者死后脑组织中 microRNA 表达谱的改变。
Biol Psychiatry. 2011 Jan 15;69(2):188-93. doi: 10.1016/j.biopsych.2010.09.039.
9
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Biol Psychiatry. 2011 Jan 15;69(2):180-7. doi: 10.1016/j.biopsych.2010.09.030. Epub 2010 Dec 15.
10
Novel approaches to the study of postmortem brain in psychiatric illness: old limitations and new challenges.研究精神疾病死后大脑的新方法：旧的局限性和新的挑战。
Biol Psychiatry. 2011 Jan 15;69(2):127-33. doi: 10.1016/j.biopsych.2010.09.035. Epub 2010 Nov 20.
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