The Zucker Hillside Hospital, Glen Oaks, NY 11004, USA.
J Mol Neurosci. 2012 Jun;47(2):243-8. doi: 10.1007/s12031-012-9731-7. Epub 2012 Mar 9.
Alterations in microRNA (miRNA) expression in postmortem brain tissue or peripheral blood have been linked to schizophrenia. Cerebrospinal fluid might provide an in vivo biomarker more directly reflecting functional changes in the brain. The goals of this study were to determine the feasibility of detecting miRNAs in cerebrospinal fluid and to compare miRNA levels in cerebrospinal fluid versus blood. Four healthy volunteers and four patients with psychotic disorders underwent a lumbar puncture and a blood draw. Expression of 378 validated miRNAs was assessed from each biofluid type for each subject using microarray technology. Five miRNAs were chosen for validation with quantitative polymerase chain reaction. A substantial number of miRNAs (n = 95) were exclusively or predominately detected in cerebrospinal fluid (CSF). Levels of 35 miRNAs detected in both CSF and blood samples in all subjects were poorly correlated. The investigation of miRNAs in CSF can help advance the understanding of psychiatric diseases and particularly schizophrenia.
脑脊髓液中的 microRNA（miRNA）表达改变与精神分裂症有关。脑脊髓液可能提供更直接反映大脑功能变化的活体生物标志物。本研究的目的是确定检测脑脊髓液中 miRNA 的可行性，并比较脑脊髓液与血液中的 miRNA 水平。4 名健康志愿者和 4 名精神病患者接受了腰椎穿刺和血液采集。使用微阵列技术从每种生物流体类型对每位受试者的 378 种经验证的 miRNA 进行表达评估。选择 5 个 miRNA 进行定量聚合酶链反应验证。大量 miRNA（n=95）仅在脑脊髓液（CSF）中检测到或主要在脑脊髓液中检测到。在所有受试者中，在 CSF 和血液样本中检测到的 35 个 miRNA 的水平相关性较差。对 CSF 中 miRNA 的研究有助于深入了解精神疾病，特别是精神分裂症。
