Gainok Jeffrey, Daniels Regina, Golembiowski David, Kindred Patricia, Post Lisa, Strickland Rob, Garrett Normalynn
Womack Army Medical Center, Fort Bragg, North Carolina, USA.
AANA J. 2011 Aug;79(4 Suppl):S28-34.
Ellagic acid (EA), a dietary supplement, is purported to have anti-inflammatory, antinociceptive properties via cyclooxygenase (COX) inhibition. We measured the antinociceptive efficacy of EA alone and in combination with a nonselective COX inhibitor and a selective COX-2 inhibitor. We assigned 54 male Sprague-Dawley rats to 1 of 6 groups to be given the following compounds: (1) vehicle, (2) ketorolac (nonselective COX inhibitor), (3) meloxicam (selective COX-2 inhibitor), (4) EA, (5) EA plus ketorolac, and (6) EA plus meloxicam. Inflammatory pain was induced in the right hind paw by injecting carrageenan. Rats were given study compounds via intraperitoneal injection 30 minutes after paw injections. Pain tolerance was assessed using the Randall-Selitto instrument at 30 minutes and 4, 8, 12, and 24 hours. The highest pressure tolerated was recorded in grams. The analysis of variance suggested a significant difference (F = 2.44; P = .048). The least significant difference post hoc analysis suggested that at 8 hours, EA plus ketorolac provided greater antinociception than all other compounds (P = .04). Furthermore the combination of EA plus ketorolac provided longer antinociception than all other compounds (P = .03) such that EA plus ketorolac was effective at 24 hours.
鞣花酸（EA）是一种膳食补充剂，据称可通过抑制环氧化酶（COX）发挥抗炎、镇痛作用。我们测定了单独使用EA以及EA与非选择性COX抑制剂和选择性COX-2抑制剂联合使用时的镇痛效果。我们将54只雄性Sprague-Dawley大鼠分为6组，分别给予以下化合物：（1）赋形剂，（2）酮咯酸（非选择性COX抑制剂），（3）美洛昔康（选择性COX-2抑制剂），（4）EA，（5）EA加酮咯酸，以及（6）EA加美洛昔康。通过注射角叉菜胶诱导右后爪出现炎性疼痛。在爪部注射后30分钟，通过腹腔注射给予大鼠受试化合物。在30分钟以及4、8、12和24小时使用Randall-Selitto仪器评估疼痛耐受性。记录耐受的最高压力，单位为克。方差分析显示存在显著差异（F = 2.44；P = .048）。最小显著差异事后分析表明，在8小时时，EA加酮咯酸的镇痛效果优于所有其他化合物（P = .04）。此外，EA加酮咯酸的镇痛持续时间比所有其他化合物更长（P = .03），以至于EA加酮咯酸在24小时时仍有效。
