Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, California, USA.
Biophys J. 2012 Mar 7;102(5):1224-33. doi: 10.1016/j.bpj.2012.01.053. Epub 2012 Mar 6.
A topographically patterned substrate with stochastic surface order that closely mimics the topographic features of native basement membranes has been fabricated to investigate the influence of topographic biophysical cueing on human aortic and umbilical vein endothelial cells. The stochastic substrate was fabricated by first generating a highly porous polyelectrolyte multilayer film of poly(acrylic acid) and poly(allylamine hydrochloride) followed by replicate production of this biomimetic topography via soft lithography. These substrates, which are easy to prepare and replicate, possess a number of prominent features associated with in vivo vascular basement membrane (interwoven ridges and grooves, bumps, and pores), which have typically been studied as singular features that frequently possess anisotropic surface order (e.g., alternating ridges and grooves). When compared to a flat surface of identical chemistry, these biomimetic topographies influenced a number of important cellular behaviors associated with the homeostasis and degradation of vascular tissues. These include modulating cell migration rate and directional persistence, proliferation rate, and gene expression associated with regulation and remodeling of vascular tissues as well as inflammation.
已经制备了具有随机表面有序的地形图案基底，其紧密模仿天然基膜的地形特征，以研究地形生物物理线索对人主动脉和脐静脉内皮细胞的影响。通过首先生成高度多孔的聚电解质多层膜聚丙烯酸和聚（盐酸烯丙胺），然后通过软光刻复制这种仿生形貌，制备了随机基底。这些基底易于制备和复制，具有许多与体内血管基底膜相关的突出特征（交织的脊和槽、凸起和孔），这些特征通常被研究为具有各向异性表面有序的单一特征（例如，交替的脊和槽）。与具有相同化学性质的平面相比，这些仿生形貌影响了与血管组织的动态平衡和降解相关的许多重要细胞行为。这些行为包括调节细胞迁移率和方向持久性、增殖率以及与血管组织的调节和重塑以及炎症相关的基因表达。
