State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Bioresour Technol. 2012 May;112:206-11. doi: 10.1016/j.biortech.2012.02.046. Epub 2012 Feb 17.
A novel cultivation strategy called "sequential heterotrophy-dilution-photoinduction" was developed for efficient algal biomass and lipid production. Three Chlorella species were first cultivated heterotrophically to achieve high cell density, then the broth was diluted to suitable concentration (2-5 g/L) and transferred to light environment for photoinduction. With this strategy, the Chlorella intracellular protein and chlorophyll increased rapidly to 50.87% and 32.97 mg/g by a 12-h illumination, which were close to the level of cells cultivated photoautotrophically. Moreover, the lipid contents were increased by 84.57%, 70.65% and 121.59% within 24-h photoinduction for C. vulgaris, C. pyrenoidosa and C. ellipsoidea, respectively. Maximum lipid content as 26.11% of biomass and maximum lipid productivity of 89.89 mg/L/d was both accomplished by C. pyrenoidosa. Further outdoor experiments showed consistent patterns. Therefore, the proposed strategy provided an effective approach for microalgal biomass production to meet the urgent need for both health food and biodiesel.
提出了一种新型的培养策略，称为“顺序异养-稀释-光诱导”，以实现高效的藻类生物质和脂质生产。首先将三种小球藻异养培养到高细胞密度，然后将培养液稀释到适当的浓度（2-5 g/L），并转移到光照环境中进行光诱导。采用该策略，小球藻胞内蛋白和叶绿素在 12 小时的光照下迅速增加到 50.87%和 32.97mg/g，接近光自养培养的细胞水平。此外，在 24 小时的光诱导下，C. vulgaris、C. pyrenoidosa 和 C. ellipsoidea 的脂质含量分别增加了 84.57%、70.65%和 121.59%。C. pyrenoidosa 的最大脂质含量达到了生物量的 26.11%，最大脂质生产力达到了 89.89mg/L/d。进一步的户外实验显示出一致的模式。因此，所提出的策略为微藻生物质生产提供了一种有效的方法，以满足人们对健康食品和生物柴油的迫切需求。
