Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, Ukraine.
Chem Phys Lipids. 2012 Apr;165(3):331-7. doi: 10.1016/j.chemphyslip.2012.02.002. Epub 2012 Mar 3.
The influence of mature lysozyme fibrils on the structural and physical properties of model membranes composed of phosphatidylcholine (PC) and its mixtures with cardiolipin (CL) (10 mol%) and cholesterol (Chol) (30 mol%) was studied using fluorescent probes DPH, pyrene, Laurdan and MBA. Analysis of pyrene fluorescence spectra along with the measurements of DPH fluorescence anisotropy revealed that the structure of hydrocarbon chains region of lipid bilayer is not affected by the fibrillar aggregates of lysozyme. In contrast, probing the membrane effects by Laurdan and MBA showed the rise of both the generalized polarization of Laurdan and the MBA fluorescence anisotropy, suggesting that amyloid protein induces reduction of bilayer hydration and increase of lipid packing in the interfacial region of model membranes.
采用荧光探针 DPH、芘、Laurdan 和 MBA 研究了成熟溶菌酶原纤维对由磷脂酰胆碱 (PC) 及其与心磷脂 (CL) (10 mol%) 和胆固醇 (Chol) (30 mol%) 的混合物组成的模型膜的结构和物理性质的影响。 芘荧光光谱的分析以及 DPH 荧光各向异性的测量表明，脂质双层的烃链区结构不受溶菌酶原纤维状聚集物的影响。 相反，通过 Laurdan 和 MBA 探测膜效应表明，Laurdan 的广义极化和 MBA 荧光各向异性均升高，表明淀粉样蛋白诱导模型膜界面区域的双层水合作用减少和脂质堆积增加。
