Department of Pathology, University of Cambridge, Cambridge, UK.
Exp Parasitol. 2012 Apr;130(4):314-20. doi: 10.1016/j.exppara.2012.02.017. Epub 2012 Mar 3.
There is definitive biochemical evidence for the presence of terminal α-galactosyl residues (α-gal) in the N-linked oligosaccharides and glycophosphatidylinositol anchors (GPI anchors) of the variant surface glycoprotein of Trypanosoma brucei bloodstream trypomastigotes. Indirect evidence also exists for α-gal in Plasmodium falciparum asexual blood stage glycoproteins and glycolipids. The occurrence of α-gal in glycoproteins and glycolipids of T. brucei bloodstream trypomastigotes and P. falciparum late asexual blood stages was investigated by the binding of α-gal-specific Bandeirea simplicifolia B4 lectin 1 (BSB4), incorporation of [(3)H]galactose from UDP-[(3)H]galactose into glycoproteins and glycolipids in microsomes in vitro, and bioinformatic searches for galactosyl-transferase coding sequences. The findings confirm the presence of α-gal in a spectrum of T. brucei bloodstream trypomastigote glycoproteins and glycolipids and indicate its relative absence from P. falciparum asexual blood stage glycoconjugates.
有明确的生化证据表明，在布氏锥虫血腔锥虫的变异表面糖蛋白的 N 连接寡糖和糖磷脂酰肌醇锚（GPI 锚）中存在末端α-半乳糖基残基（α-gal）。间接证据也表明恶性疟原虫无性血期糖蛋白和糖脂中存在α-gal。通过α-gal 特异性 Bandeirea simplicifolia B4 凝集素 1（BSB4）的结合、在体外微体中从 UDP-[3H]半乳糖掺入[3H]半乳糖到糖蛋白和糖脂中以及生物信息学搜索半乳糖基转移酶编码序列，研究了布氏锥虫血腔锥虫和恶性疟原虫晚期无性血期糖蛋白和糖脂中α-gal 的存在情况。研究结果证实了α-gal 在一系列布氏锥虫血腔锥虫糖蛋白和糖脂中的存在，并表明其在恶性疟原虫无性血期糖缀合物中相对不存在。
