Mycology and Botany Program, American Type Culture Collection, 10801 University Blvd., Manassas, VA 20110, USA.
Antonie Van Leeuwenhoek. 2012 Jun;102(1):99-104. doi: 10.1007/s10482-012-9717-z. Epub 2012 Mar 10.
Two strains of a basidiomycetous yeast were derived from an insect trypanosomatid culture isolated from the intestine of a plant bug, Collaria oleosa (Heteroptera: Miridae), collected in Costa Rica. The yeast did not form ballistoconidia but reproduced only by budding. Teliospores were not observed in individual and crossed cultures of each strain. Morphological and other taxonomic characteristics of the yeast were similar to those of the species in the polyphyletic genus Rhodotorula. However, molecular phylogeny inferred from the internal transcribed spacers and D1/D2 region of the large subunit rRNA gene showed that the strains represent a new species placed among the smut fungi in the family Ustilentylomataceae, which includes Aurantiosporium subnitens, Fulvisporium restifaciens, Ustilentyloma fluitans, and Rhodotorula hordea. Given the well distinguished phylogenetic position of this novel species within the Ustilentylomataceae, we propose Microbotryozyma collariae gen. nov., sp. nov. to accommodate the yeast isolated from C. oleosa, with strain American Type Culture Collection MYA-4666(T) (= PRA303-1S = CBS 12537) designated as the type strain.
两株担子菌酵母菌是从一种昆虫原生动物培养物中分离出来的，该培养物来自于哥斯达黎加采集的一种植物蝽 Collaria oleosa（半翅目：Miridae）的肠道。酵母不形成掷孢子，但只能通过出芽繁殖。在每个菌株的单独和杂交培养中都未观察到厚垣孢子。酵母的形态和其他分类特征与多系属罗地毛霉属的物种相似。然而，从内部转录间隔区和大亚基 rRNA 基因的 D1/D2 区推断的分子系统发育表明，这些菌株代表了一种新物种，它位于黑粉菌科 Ustilentylomataceae 中的 smut 真菌中，其中包括 Aurantiosporium subnitens、Fulvisporium restifaciens、Ustilentyloma fluitans 和 Rhodotorula hordea。鉴于该新型物种在 Ustilentylomataceae 内的明显进化地位，我们提出了 Microbotryozyma collariae gen. nov.，sp. nov. 以容纳从 C. oleosa 中分离出的酵母，其中菌株 American Type Culture Collection MYA-4666(T) (= PRA303-1S = CBS 12537)被指定为模式株。
