Department of Pharmacology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Eur Addict Res. 2012;18(4):161-6. doi: 10.1159/000336314. Epub 2012 Mar 9.
Alcohol dependence causes serious problems which may be influenced by genetic factors associated with alcohol metabolism. The aim was to investigate the allelic and genotypic difference in distribution of a polymorphism in alcohol dehydrogenase 1C gene (ADH1C) between alcohol-dependent individuals and controls, and to examine if these genotypes were associated with the age at which the patient became alcohol-dependent.
We conducted a case-control study including 90 alcohol-dependent cases and 100 historic controls. The genomic DNA was isolated and the alleles were analyzed with an RFLP.
The ADH1C1 allele frequencies were 0.89 (95% CI 0.84-0.91) in controls and 0.68 (95% CI 0.61-0.74) in alcohol-dependent patients. The frequencies of the ADH1C2 allele were 0.11 (95% CI 0.07-0.14) and 0.32 (95% CI 0.25-0.38) among controls and alcohol-dependent patients, respectively (p < 0.0001). The ADH1C*1/1 genotype frequency was significantly higher in the control group (77%) compared to that of the alcohol-dependents (51%, p < 0.0001). The ADH1C1/*2 genotype frequency was significantly lower in the control group (23%) compared to that of the alcohol-dependents (42%, p < 0.0001). We obtained no statistically significant difference among the ADH1C genotype groups regarding age.
These findings suggest that a significantly higher presence of ADH1C*2 allele is associated with alcohol dependence in a Turkish population. Studies with other related polymorphisms are needed to more precisely estimate the association of alcohol dependence with ADH1C.
酒精依赖会导致严重问题，这些问题可能受到与酒精代谢相关的遗传因素的影响。本研究旨在探讨酒精脱氢酶 1C 基因（ADH1C）多态性在酒精依赖患者和对照组中的等位基因和基因型分布差异，并探讨这些基因型是否与患者开始饮酒的年龄有关。
我们进行了一项病例对照研究，纳入了 90 例酒精依赖患者和 100 例历史对照。提取基因组 DNA，采用 RFLP 分析等位基因。
对照组 ADH1C1 等位基因频率为 0.89（95%CI 0.84-0.91），酒精依赖患者为 0.68（95%CI 0.61-0.74）。ADH1C2 等位基因频率分别为对照组 0.11（95%CI 0.07-0.14）和酒精依赖患者 0.32（95%CI 0.25-0.38）（p<0.0001）。对照组 ADH1C*1/1 基因型频率明显高于酒精依赖患者（77%比 51%，p<0.0001）。对照组 ADH1C1/*2 基因型频率明显低于酒精依赖患者（23%比 42%，p<0.0001）。ADH1C 基因型组间年龄无统计学差异。
这些发现表明，土耳其人群中 ADH1C*2 等位基因的存在显著增加与酒精依赖有关。需要进行其他相关多态性的研究，以更准确地评估 ADH1C 与酒精依赖的关联。
