Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taichung, Taiwan.
J Mater Sci Mater Med. 2012 Jun;23(6):1465-78. doi: 10.1007/s10856-012-4608-4. Epub 2012 Mar 14.
This work developed a novel bi-layer wound dressing composed of 3D activated carbon fibers that allows facilitates fibroblast cell growth and migration to a wound site for tissue reconstruction, and the gentamicin is incorporated into a poly(γ-glutamic acid)/gelatin membrane to prevent bacterial infection. In an in vitro, field emission scanning electron microscopy shows that rat skin fibroblasts appeared and spread on the surface of activated carbon fibers, and penetrated the interior and exterior of the 3D activated carbon fiber construct to a depth of roughly 200 μm. An in vivo analysis shows that fibroblast cells containing the proposed 3D scaffold had the potential of a biologically functionalized dressing to accelerate wound closure. Additionally, fibroblasts migrated to the wound site in a bi-layer wound dressing containing fibroblasts, enhancing fibronectin and type I collagen expression, resulting in faster skin regeneration than that achieved with a Tegaderm™ hydrocolloid dressing or gauze.
这项工作开发了一种新型双层创面敷料，由允许促进成纤维细胞生长和迁移到创面进行组织重建的 3D 活性炭纤维组成，并且将庆大霉素掺入聚（γ-谷氨酸）/明胶膜中以防止细菌感染。在体外，场发射扫描电子显微镜显示，大鼠皮肤成纤维细胞出现在活性炭纤维表面并在其上扩散，并且渗透到 3D 活性炭纤维结构的内外，深度约为 200μm。体内分析表明，含有所提出的 3D 支架的成纤维细胞具有生物功能化敷料的潜力，可加速伤口闭合。此外，在含有成纤维细胞的双层创面敷料中，成纤维细胞迁移到创面部位，增强了纤连蛋白和 I 型胶原蛋白的表达，使皮肤再生速度快于 Tegaderm™水胶体敷料或纱布。
