Josephi E, Braito U, Tutsch-Bauer E
Institut für Rechtsmedizin, Universität München.
Beitr Gerichtl Med. 1990;48:1-5.
DNA of 1 ml Ascites, bile, liquor, pleural-exsudate, pericardial-fluid, synovial-fluid, urine and vitreous humor of sixteen bodies of either sex was isolated. Applying dot-blot-hybridisation black spots were obtained by using total human DNA as DNA-probe (A), if the origin is human. Our results showed, that body-fluids of dead persons contain human DNA, mainly. After rehybridisation with a sex-specific DNA-probe (Y) (pJA 1143) a determination of sex can be performed.
从16具不同性别人体的1毫升腹水、胆汁、脑脊液、胸腔渗出液、心包液、滑液、尿液和玻璃体液中分离出DNA。应用斑点杂交技术，如果样本来源是人，以人总DNA作为DNA探针（A）可得到黑色斑点。我们的结果表明，死者的体液主要含有人类DNA。在用性别特异性DNA探针（Y）（pJA 1143）重新杂交后，可以进行性别鉴定。
