Rosset Emilie, Rannou Benoit, Casseleux Grégory, Chalvet-Monfray Karine, Buff Samuel
Centre d'Étude et de Recherche en Reproduction et en Élevage Canin-CERREC, Université de Lyon, VetAgro Sup, Campus Vétérinaire de Lyon, 1 Avenue Bourgelat, 69280 Marcy l’Étoile, France
Vet Clin Pathol. 2012 Jun;41(2):272-82. doi: 10.1111/j.1939-165X.2012.00415.x. Epub 2012 Mar 15.
Nonspecific signs of illness and availability of only limited information about expected biochemical and hematologic values in puppies make formulation of a differential diagnosis challenging for veterinarians.
The objectives of this prospective study were to describe age-related changes in biochemical and hematologic variables in Borzoi and Beagle puppies from birth to 8 weeks and to compare results with adult reference intervals (RIs).
Biochemical and hematologic analytes in blood collected in heparin and EDTA from 31 clinically healthy puppies (21 Beagle and 10 Borzoi dogs) were measured or calculated each week from birth to 8 weeks of age. RIs were calculated using the parametric robust method after Box-Cox transformation.
There were no significant differences between values of puppies and adults for ALT activity, glucose concentration, MCHC, WBC count, and platelet count. Total protein concentration, RBC count, and HCT were lower and creatine kinase activity was higher in puppies of all ages compared with these variables in adults. Compared with adult RI, in puppies urea concentration was higher at birth and week 1, creatinine concentration was lower at weeks 1-5, ALP activity was higher at all weeks except for weeks 4 and 6, hemoglobin concentration was lower at weeks 1-8, MCV was higher at birth and week 1, and reticulocyte count was higher at all weeks except week 1. Statistically, mixed models accounted for evaluation of the same puppies each week and in most cases demonstrated an effect of age and sometimes breed. Some reference intervals should be used with caution owing to potential outliers. The 90% confidence intervals exceeded those recommended by the International Federation of Clinical Chemistry.
Clinicians should be aware of temporal changes in biochemical and hematologic values for puppies during the first 2 months of life to avoid misinterpretation of results.
幼犬疾病的非特异性体征以及关于预期生化和血液学值的信息有限，这使得兽医进行鉴别诊断具有挑战性。
这项前瞻性研究的目的是描述波索尔犬和比格犬幼犬从出生到8周龄时生化和血液学变量的年龄相关变化，并将结果与成年犬的参考区间（RIs）进行比较。
从31只临床健康的幼犬（21只比格犬和10只波索尔犬）采集肝素和乙二胺四乙酸抗凝的血液，从出生到8周龄每周测量或计算生化和血液学分析物。采用Box-Cox变换后的参数稳健方法计算参考区间。
幼犬和成年犬在谷丙转氨酶活性、葡萄糖浓度、平均血红蛋白浓度、白细胞计数和血小板计数方面的值无显著差异。与成年犬相比，各年龄段幼犬的总蛋白浓度、红细胞计数和血细胞比容较低，肌酸激酶活性较高。与成年犬参考区间相比，幼犬出生时和第1周尿素浓度较高，第1 - 5周肌酐浓度较低，除第4周和第6周外所有周碱性磷酸酶活性较高，第1 - 8周血红蛋白浓度较低，出生时和第1周平均红细胞体积较高，除第1周外所有周网织红细胞计数较高。统计学上，混合模型考虑了每周对同一只幼犬的评估，在大多数情况下显示出年龄以及有时品种的影响。由于存在潜在的异常值，一些参考区间应谨慎使用。90%置信区间超过了国际临床化学联合会推荐的范围。
临床医生应了解幼犬出生后前2个月生化和血液学值的时间变化，以避免对结果的错误解读。
