Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar, 243122 Uttar Pradesh, India.
Parasitol Res. 2012 Aug;111(2):629-35. doi: 10.1007/s00436-012-2879-9. Epub 2012 Mar 16.
With an aim to evaluate the protective potentiality of rBm86 against Rhipicephalus (Boophilus) microplus Indian Veterinary Research Institute (IVRI)-I line and Hyalomma anatolicum anatolicum IVRI-II line infestations on crossbred (Bos indicus × Bos taurus) calves, 20 animals of 3 months of age were randomly divided in to four equal groups and maintained in tick-proof conditions. Animals of groups 1 and 2 were immunized with 2 ml of rBm86 (100 μg)-based vaccine (procured from Revetmex S.A. de C.V, Mexico City, Mexico) thrice at 30 days interval. Animals of groups 3 and 4 were kept as negative control and inoculated with PBS only. Each animal of group 1 and 3 was challenged with 7-day-old 50 unfed adults of H. anatolicum anatolicum (1:1, male and female), and each animal of groups 2 and 4 was challenged with 6-8-day-old R. (B.) microplus larvae obtained from 50 mg of eggs, on 17th day of the last immunization. The efficacy of rBm86 against tick infestations was determined as percentage reduction in number of adults dropped (DT%), engorged body weight (DR%), egg masses (DO%), and immunogen efficacy (E%). The calculated data were 11.8, 10.8, 15.0, and 25.1 %, respectively, for DT, DR, DO, and E% against H. anatolicum anatolicum infestation, while in the case of R. (B.) microplus infestation, the corresponding data were 6.4, 11.24, 40.7, and 44.5 %, respectively. The results indicated partial effectiveness of rBm86 antigen(s) in imparting protection against homologous and heterologous challenge infestations of Indian ticks. The results indicated identification of more effective antigen(s) for the development of vaccine against economically important tick species in India.
为了评估 rBm86 对印度兽医研究所（IVRI）-I 系的 Rhipicephalus（Boophilus）microplus 和 IVRI-II 系的 Hyalomma anatolicum anatolicum 感染的保护潜力，将 20 头 3 月龄的杂交（Bos indicus × Bos taurus）小牛随机分为四组，每组 5 头，置于防蜱环境中。第 1 组和第 2 组动物用 2 ml rBm86（100 μg）疫苗（购自墨西哥城 Revetmex S.A. de C.V.）免疫 3 次，间隔 30 天。第 3 组和第 4 组动物作为阴性对照，仅接种 PBS。第 1 组和第 3 组的每只动物用 7 日龄、50 只未饱血的 H. anatolicum anatolicum （雌雄比 1:1）进行攻虫，第 2 组和第 4 组的每只动物用 6-8 日龄、从 50mg 卵中获得的 R.（B.）microplus 幼虫进行攻虫，在最后一次免疫后第 17 天进行。rBm86 对蜱感染的疗效通过减少掉落的成虫数（DT%）、饱食体重（DR%）、卵团（DO%）和免疫原性（E%）来确定。计算出的 DT、DR、DO 和 E%对 H. anatolicum anatolicum 感染的分别为 11.8%、10.8%、15.0%和 25.1%，而对 R.（B.）microplus 感染的相应数据分别为 6.4%、11.24%、40.7%和 44.5%。结果表明 rBm86 抗原（s）在赋予同源和异源挑战感染印度蜱的保护方面具有部分有效性。这些结果表明，需要鉴定更有效的抗原（s）来开发针对印度具有经济重要性的蜱种的疫苗。
