Miranpuri G S
Division of Parasitology, Indian Veterinary Research Institute, U.P.
Vet Parasitol. 1989 Jun;31(3-4):289-301. doi: 10.1016/0304-4017(89)90079-4.
Studies were undertaken to determine the effect of repeated pure infestations with Boophilus microplus on susceptibility to subsequent pure infestations with Hyalomma anatolicum anatolicum, and the effects of pure infestations with both species of tick on susceptibility to a series of mixed infestations. Crossbred (Bos indicus X Bos taurus) calves were infested with Boophilus microplus (17 times), H. a. anatolicum (four times), followed by five mixed infestations of B. microplus and H. a. anatolicum. The decline in B. microplus engorgement from a mean yield of 274.4 +/- 60.3 ticks per host after the first exposure, to a mean yield of 9 +/- 4.6 per animal after the seventeenth exposure, was observed in animals exposed to only B. microplus. This might be due to acquired resistance. However, these animals were found to be as susceptible to H. a. anatolicum as animals which had never been exposed to ticks of either species. A decline in the yield of H. a. anatolicum from a mean yield of 92.1 +/- 10.7 after the first exposure to 54.7 +/- 11.3 after the fourth exposure, indicated that the cattle could also acquire resistance to repeated pure infestations with this species. After repeated pure infestations with both tick species, cattle reacted to five mixed infestations showing a high degree of resistance to B. microplus and low resistance to H. a. anatolicum (mean yield for B. microplus was only 10 +/- 8.1 ticks per host after the first mixed exposure and declined to 1.3 +/- 1.7 after the fifth, whereas the mean yield for H. a. anatolicum was 71.4 +/- 11.3 ticks per host following the first exposure and declined to 37.3 +/- 7.8 after the fifth). Host responses elicited to one species do not provide cross-resistance to the second species used in this study.
开展了多项研究，以确定微小牛蜱的反复单纯感染对随后安纳托利亚璃眼蜱单纯感染易感性的影响，以及这两种蜱的单纯感染对一系列混合感染易感性的影响。对杂交（印度瘤牛×普通牛）犊牛进行微小牛蜱感染（17次）、安纳托利亚璃眼蜱感染（4次），随后进行微小牛蜱和安纳托利亚璃眼蜱的5次混合感染。在仅接触微小牛蜱的动物中，观察到微小牛蜱饱血数量从首次接触后每头宿主平均产蜱274.4±60.3只，下降到第17次接触后每头动物平均产蜱9±4.6只。这可能是由于获得性抗性。然而，发现这些动物对安纳托利亚璃眼蜱的易感性与从未接触过任何一种蜱的动物相同。安纳托利亚璃眼蜱的产蜱数量从首次接触后的平均92.1±10.7只下降到第4次接触后的54.7±11.3只，表明牛对该物种的反复单纯感染也能获得抗性。在这两种蜱都进行反复单纯感染后，牛对5次混合感染有反应，对微小牛蜱表现出高度抗性，对安纳托利亚璃眼蜱抗性较低（首次混合接触后微小牛蜱每头宿主平均产蜱仅为10±8.1只，第5次接触后降至1.3±1.7只，而安纳托利亚璃眼蜱首次接触后每头宿主平均产蜱为71.4±11.3只，第5次接触后降至37.3±7.8只）。对一种蜱产生的宿主反应并不能为本研究中使用的第二种蜱提供交叉抗性。
