Kruczek Michał, Swiderski Adam, Mech-Nowak Aleksandra, Król Katarzyna
Department of Biochemistry, University of Agriculture in Kraków, Poland.
Acta Biochim Pol. 2012;59(1):135-7. Epub 2012 Mar 17.
Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.
采用FRAP法和DPPH法对从沙棘果实中提取的含类胡萝卜素提取物进行抗氧化能力的比较分析。该检测包括在比较栽培中生长的9个沙棘品种。进行的分析能够将抗氧化能力与通过分光光度法和HPLC法测定的类胡萝卜素含量进行比较。在FRAP法和DPPH法中均显示出高抗氧化活性的3个受试品种,在HPLC分析中也显示出最高(“Aromatnaya”)和高(“Botanicheskaya”、“Arumnyj”)的总类胡萝卜素含量。