Teleszko Mirosława, Wojdyło Aneta, Rudzińska Magdalena, Oszmiański Jan, Golis Tomasz
†Department of Fruit and Vegetable Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37 Street, 51 630 Wrocław, Poland.
‡Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31 Street, 60 624 Poznań, Poland.
J Agric Food Chem. 2015 Apr 29;63(16):4120-9. doi: 10.1021/acs.jafc.5b00564. Epub 2015 Apr 20.
The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophaë rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major polyphenol group identified in berries was flavonols (mean content of 311.55 mg/100 g fw), with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p < 0.05).
本研究的目的是测定8个沙棘（沙棘蒙古亚种）品种果实中的某些植物化学物质，包括亲脂性和亲水性化合物。在实验中，采用了气相色谱法（用于分析植物甾醇和脂肪酸）、超高效液相色谱-光电二极管阵列-荧光检测法、液相色谱-质谱联用法（用于分析多酚）、高效液相色谱法（用于分析L-抗坏血酸）以及分光光度法（用于分析总类胡萝卜素）。从整个果实中分离出的脂质部分含有14种植物甾醇（主要成分β-谷甾醇>24-亚甲基环阿尔廷醇>角鲨烯）和11种脂肪酸，其顺序为单不饱和脂肪酸>饱和脂肪酸>多不饱和脂肪酸。类胡萝卜素的含量在6.19至23.91毫克/100克鲜重（fw）之间（p<0.05）。浆果中鉴定出的主要多酚类是黄酮醇（平均含量为311.55毫克/100克fw），其结构为异鼠李素（6种化合物）、槲皮素（4种化合物）和山奈酚（1种化合物）的糖苷。所检测的沙棘品种还具有较高含量的L-抗坏血酸，范围为52.86至130.97毫克/100克fw（p<0.05）。
