Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM. FF. NN, Università di Messina, Contrada Papardo, Salita Sperone 31, 98166 Messina, Italy.
Phytochem Anal. 2012 May-Jun;23(3):267-73. doi: 10.1002/pca.1353. Epub 2011 Aug 18.
The berries of Hippophae rhamnoides Linnaeus have high nutritional and medicinal values and have been used for centuries as food both in Europe and Asia. The oleoresins represent a potential source of carotenoid esters and can be used as food additives, cosmetic ingredients or nutraceuticals.
The objective of this study was to develop a HPLC-DAD-APCI-MS method, with both positive and negative ionisation modes, for the direct identification of the native carotenoid composition in fruits of Hippophae rhamnoides.
Fruits of Hippophae rhamnoides, cv. Serbanesti and Victoria, were collected from an experimental field at the Fruit Research Station of Bacau, Romania. Samples were extracted using methanol:ethyl acetate:petroleum ether (1:1:1, v/v/v). The HPLC-DAD-APCI-MS analyses were carried out on a Shimadzu system using a YMC C₃₀-column and a gradient elution.
In total 22 compounds were detected, eight were free carotenoids, nine were xanthophylls monoesters and five were xanthophylls diesters. Differences were observed in the relative percentage composition of the identified components among the two cultivars investigated. Zeaxanthin-C16:0,C16:0 was the most abundant diester. The unsaturated palmitoleic acid was directly detected in its esterified form, in zeaxanthin-C16:0,C16:1, which is reported here for the first time. Although present in small amounts the unsaturated oleic, linoleic, linolenic, hexadecadienoic and hexadecatrienoic acids were detected in their esterified forms as lutein monoesters, the last two having been detected in Hippophae rhamnoides for the first time.
A novel (HPLC-DAD-APCI-MS) method was developed for the direct identification of the native carotenoid composition in fruits of Hippophae rhamnoides.
沙棘浆果具有很高的营养价值和药用价值，在欧洲和亚洲，几个世纪以来，它们一直被用作食物。其油树脂是类胡萝卜素酯的潜在来源，可用作食品添加剂、化妆品成分或营养保健品。
本研究旨在建立一种高效液相色谱-二极管阵列检测-大气压化学电离-质谱联用（HPLC-DAD-APCI-MS）方法，采用正、负离子化模式，直接鉴定沙棘果实中的天然类胡萝卜素组成。
从罗马尼亚巴克乌水果研究站的实验田中采集沙棘（Hippophae rhamnoides）品种 Serbanesti 和 Victoria 的果实。采用甲醇：乙酸乙酯：石油醚（1:1:1，v/v/v）提取样品。HPLC-DAD-APCI-MS 分析在 Shimadzu 系统上进行，采用 YMC C₃₀ 柱和梯度洗脱。
共检测到 22 种化合物，其中 8 种为游离类胡萝卜素，9 种为叶黄素单酯，5 种为叶黄素二酯。在所研究的两个品种中，鉴定成分的相对百分比组成存在差异。玉米黄质-C16:0，C16:0 是最丰富的二酯。棕榈油酸以其酯化形式，即玉米黄质-C16:0，C16:1 直接检测到，这是首次报道。尽管含量较少，但不饱和的油酸、亚油酸、亚麻酸、十六碳二烯酸和十六碳三烯酸以叶黄素单酯的形式检测到，后两者在沙棘中首次检测到。
建立了一种用于直接鉴定沙棘果实中天然类胡萝卜素组成的新方法（HPLC-DAD-APCI-MS）。
