Kondracki Stanisław, Banaszewska Dorota, Wysokńjska Anna, Iwanina Maria
Department of Animal Reproduction and Hygiene, Institute of Bioengineering and Animal Breeding, University of a Natural Sciences and Humanities in Siedlce, Prusa 14, 08-110 Siedlce, Poland.
Folia Biol (Krakow). 2012;60(1-2):85-91. doi: 10.3409/fb60_1-2.85-91.
Experiments were performed on 75 ejaculates obtained from 19 bulls representing different cattle breeds used at the Masovian Centre for Animal Breeding and Reproduction in Łowicz. Fresh ejaculates were measured in respect to their volume and sperm count in the ejaculates was determined. The ejaculates were classified based on the criterion of sperm concentration and divided into five groups. Sperm morphometric measurements were taken from each bull and assessment of semen morphology was done on the basis of examination under a microscope using preparations made from fresh ejaculates. For each slide, morphometric measurements were taken of 15 randomly selected spermatozoa characterised by normal morphology and well visible under the microscope. Additionally, in each preparation morphometry of 500 spermatozoa was evaluated, numbers of spermatozoa with normal morphology and morphological abnormalities were recorded and these were categorized into spermatozoa with major and minor defects. An insignificant correlation was observed between the sperm concentration in the ejaculate and morphological traits, dimensions and shapes of bull spermatozoa. The less concentrated ejaculates contained spermatozoa with a slightly larger head circumference and a more elongated head shape in comparison with the spermatozoa in the more concentrated ejaculates. The highest frequency of morphologically malformed spermatozoa, both in the case of primary and secondary alterations, was observed in ejaculates with sperm concentration of no more than 1000 x 10(3)/mm3.
对从罗维茨马佐夫舍动物育种与繁殖中心使用的代表不同牛品种的19头公牛采集的75份精液进行了实验。对新鲜精液的体积进行了测量，并测定了精液中的精子数量。根据精子浓度标准对精液进行分类，分为五组。对每头公牛进行精子形态测量，并基于使用新鲜精液制成的制剂在显微镜下检查的结果对精液形态进行评估。对于每张载玻片，对15个随机选择的形态正常且在显微镜下清晰可见的精子进行形态测量。此外，在每个制剂中评估500个精子的形态学，记录形态正常和形态异常的精子数量，并将这些精子分类为有主要缺陷和次要缺陷的精子。观察到精液中的精子浓度与公牛精子的形态特征、尺寸和形状之间存在不显著的相关性。与浓度较高的精液中的精子相比，浓度较低的精液中的精子头部周长略大，头部形状更细长。在精子浓度不超过1000×10(3)/mm3的精液中，无论是原发性还是继发性改变，形态异常精子的频率最高。
