Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan, China.
FEMS Microbiol Lett. 2012 Jun;331(1):37-43. doi: 10.1111/j.1574-6968.2012.02548.x. Epub 2012 Apr 17.
Small non-coding RNAs (sRNAs) are important post-transcriptional regulators in prokaryotes and have been demonstrated to participate in most of the cellular processes. SraG is an sRNA found in several enterobacterial species, but its targets have not been characterized. Here, we compared the protein expression patterns between the wild-type and an sraG-depleted mutant of Yersinia pseudotuberculosis by proteomic analysis. Sixteen proteins were up- or downregulated, and the negative regulatory role of SraG associated with the YPK_1206-1205 operon was confirmed. A region in the coding sequence of YPK_1206 was further demonstrated to be required for this negative regulation.
小非编码 RNA（sRNAs）是原核生物中重要的转录后调控因子，已被证明参与了大多数细胞过程。SraG 是一种在几种肠杆菌物种中发现的 sRNA，但它的靶标尚未被表征。在这里，我们通过蛋白质组学分析比较了野生型和耶尔森氏菌 sraG 缺失突变体之间的蛋白质表达模式。有 16 种蛋白质被上调或下调，SraG 与 YPK_1206-1205 操纵子的负调控作用得到了证实。进一步证实，YPK_1206 的编码序列中的一个区域是这种负调控所必需的。
