Electrical Engineering Department, University of California, Los Angeles, USA.
Anal Cell Pathol (Amst). 2012;35(4):229-47. doi: 10.3233/ACP-2012-0057.
The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity. Lensfree computational microscopy is rapidly emerging toward this end, and by discarding lenses and other bulky optical components of conventional imaging systems, and relying on digital computation instead, it can achieve both reflection and transmission mode microscopy over a large field-of-view within compact, cost-effective and mechanically robust architectures. Such high throughput and miniaturized imaging devices can provide a complementary toolset for telemedicine applications and point-of-care diagnostics by facilitating complex and critical tasks such as cytometry and microscopic analysis of e.g., blood smears, Pap tests and tissue samples. In this article, the basics of these lensfree microscopy modalities will be reviewed, and their clinically relevant applications will be discussed.
近年来，数字技术和信息处理方法的革命为改变光学成像方式带来了重要机遇，特别是在提高显微镜的通量的同时降低其相对成本和复杂性。无透镜计算显微镜正是朝着这个方向迅速发展的，它摒弃了传统成像系统中的透镜和其他大型光学元件，转而依靠数字计算，从而可以在紧凑、经济高效和机械坚固的架构内实现大视场的反射和透射模式显微镜。这种高吞吐量和微型化的成像设备可以通过促进细胞术和例如血液涂片、巴氏涂片和组织样本的微观分析等复杂和关键任务，为远程医疗应用和即时诊断提供补充工具集。本文将回顾这些无透镜显微镜模式的基础知识，并讨论它们在临床相关应用中的情况。
